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上海玉博生物科技有限公司
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文献和实验Add 700ul ml sterile PEG solution (40 % PEG, 1 X TE, 1 XLiAC, made fresh from stocks of 50 % PEG 4000, 10 X TE, 10 X LiAC) to each tube and incubate at 30°C for 45min. up to 1.5hrs (longer is fine if maximum efficiency is not required; 1.5hrs
Applications of Modified Transcripts
of active, highly modified RNAs. In special cases, the enzymatic synthesis of highly modified ribozymes is possible. For example, in the synthesis of hammerhead ribozymes, CTP, GTP, and UTP can be substituted by the corresponding NTPαS (2 ). Furthermore
Incorporation of Modified Bases into Oligonucleotides
) and metal-chelating groups. Electrophilic and photochemical alkylating functions have been attached to react with the other strand(s) in a duplex (or triplex).
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