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文献和实验Protocols for the preparation of tumour cells for s.c. injections in mice
, 1% antibiotic solution (penicillin, streptomycin, fungizone) and filtered glucose (0.37%). Flasks: Small-size flasks (25 cm2 ) are filled with 8-10 ml of medium. Medium-size flasks (75 cm2 ) are filled with 20 ml of medium. Large-size flasks (150 cm
Rapid Extraction of High Quality DNA from Whole Blood Stored at 4ºC for Long Period
100. Add 10 ml of 1 M Tris, 109.54 gr of sucrose, 1.01 gr MgC12, adjust pH to 8.0 and finally add 10 ml of Triton X-100 to 800 ml of distilled water, and make up to 1 liter with distilled water. Autoclave at 15 p.s.i. for 10 min. Sugars at high
The Effect of Caffeine on the Heart Rates of Three-Day Old Chick Embryos
of caffeine to which the heart was exposed. Procedure Preparation: Prep sheet 1) Warm Dulbecco's modified Eagle Medium (DMEM, Sigma) and Howard's Ringers solution to 37 degrees C. Diliute the 1 mg/ml stock caffeine solution in DMEM. 2) Pour
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