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文献和实验nmGFPGreen395/475 nm509 nmCy3Yellow548 nm562 nmCy3BYellow558 nm572 nmAlexa Fluor 532Yellow531 nm554 nmAlexa Fluor 546Yellow556 nm573 nmAlexa Fluor 555Yellow555 nm565 nmR-PhycoerythrinYellow480, 546, 565 nm578 nmB-PhycoerythrinYellow546, 565 nm575 nmHNP/Fast
Use of Flow Cytometric Methods to Quantify Protein‐Protein Interactions
Introduction Characterizing Protein‐Protein Binding Using the Flow Cytometry Protein‐Protein Interaction Assay Basic Protocol 1: Saturation Analysis of Biotin‐RGS with Alexa Fluor532‐Gαo
Coupling Peptides To Resin For Affinity Purification
5% ethylene diamine in water. Incubate 15' at RT. Wash with 10 volumes water. At this point you have amino-affigel. Wash with 3 volumes of 0.1 M NaPi pH 7.8. Resuspend resin in 0.1 M NaPi pH 7.8. Add iodoacetic acid-NHS ester, and incubate
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