thermo特约代理商|AB0576 Gene Frame,

Clonality - X Chromosome Inactivation Assay

) Forward and reverse primers, each 20 µM (Human Androgen Receptor Gene (HUMARA) CAG trinucleotide repeat microsatellite primers are described in Allen et al) AmpliTaq Gold, 5 U/ml (Perkin Elmer) a 32P-dCTP, 20 µCi/µl (NEN) Gel-Mix 6 (Life Technologies

Quantification of Gene Transcripts with Deep Sequencing Analysis of Gene Expression (DSAGE) Using 1 to 2 µg Total RNA

analysis of gene expression (DSAGE) measures global gene transcript levels from only 1 to 2 µg total RNA by massively parallel sequencing of cDNA tags. This unit describes the construction of 21?bp cDNA tag libraries appropriate for massively parallel

【转帖】7cm/11cm/17cm IPG胶条等电聚焦

 8-10  20%  5ul  25ul  250ul 3. 从小管中取出 400 m l 水化上样缓冲液，加入 100 m l 样品，充分混匀。其中水化缓冲液与样品的比例不得小于 4:1 。具体的上样体积如下表， ReadyStrip TM IPG胶条的长度  上样体积 7 cm  125ul-250ul 11cm  185ul-370ul 17cm  300ul-600ul 其中蛋白质的上样量与染色相关。具体的蛋白质上样量如下表， IPG 胶条的长度  分析型的上样