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文献和实验LABELING MUSCLE ACTIN WITH CARBOXYFLUORESCEIN SUCCINIMIDYL ESTER
,50Ti tubes,small vial. 8.G-25-150 column,~30x1.5 cm. Procedure (perform under reduced light,4℃ unless otherwise noted) 1.Resuspend 10 mg lyophilized actin in 2 ml buffer 1.Be careful not to make bubbles. 2.Add DTT to 0.5 mM. 3.Dialyze against 250
Preparation of Carboxypeptidase Y and Its Properties
⑺ 1mmol/L N-acetyl-L-tyrosine ethyl ester(ATEE): Weigh 6.25 mg of CBZ-Phe-Leu, dissolve it into 0.05mol/L pH7.5 phosphate buffer solution and set the volume at 25ml. ⑻ Nessler's reagent: Dissolve 10g hydrargyri iodidum
LABELING GC-GLOBULIN WITH FLUORESCEIN
Materials 1.2 mM PIPES,pH 7.0,500 ml. 2.Gc-globulin (Calbiochem 345802),1 mg. 3.CFSE (Carboxyfluorescein succinimidyl ester; Molecular Probes). 4.Lysine,100 mM in buffer 1,10 ml. 5.G25-150 desalting column,1 cm diameter.Equilibrated with buffer
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