vectorlabs特约代理商|SK-4103 ImmPAC

IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Diaminobenzadine (DAB) brown detection

from Vector DAB substrate kit (Catalog No. SK-4100), four drops DAB, two drops H2O2 , and two drops of Nickel from kit, in 15ml tube (wrap the tube in foil to protect the substrate from light) . Incubate in the dark for 20-30 min., stop color reaction

免疫细胞化学常用试剂

浸泡固定2~24h。另外，该固定剂较为温和，适于组织标本的较长期保存。2、4%多聚甲醛-磷酸二氢钠/氢氧化钠试剂：A液：多聚甲醛40g蒸馏水 400mlB液：Na2HPO4•2H2O16.88g蒸馏水 300mlC液：NaOH 3.86g蒸馏水 200m配制方法：A液最好在500ml的三角烧瓶中配制（方法同前），至多聚甲醛完全溶解后冷却待用。注意，在溶解多聚甲醛时，要尽量避免吸入气体或溅入眼内。B液和C液配制好后，将B液倒入C液中，混合后再加入A液，以1n NaOH或1N HCl 将pH调至

IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Diaminobenzadine

. each in 1x PBS, followed by 1 wash in 100mM Tris pH 8.2 for 5 min. Make up substrate solution immediately before use. Add 5 ml of sterile water, two drops of buffer from Vector DAB substrate kit (Catalog No. SK-4100), four drops DAB, two drops H2