vectorlabs特约代理商|SK-4100 DAB SU

IMMUNOHISTOCHEMISTRY on PARAFFIN OR CRYOSECTIONS

for Peroxidase [VectorLabs #SK4100]: 5 mL dH2O 2 drops DAB substrate buffer. Mix well 4 drops DAB. Mix well 2 drops H2O2. Mix well 2 drops nickel-cobalt solution. Mix well a) use immediately upon making solution, 2-10 min usually enough to stain b) stop

All-in-One Kit for Immunohistochemical Staining for Tissues with Antibodies

0.05% Tween-20 for 2 minutes, each time. 4)   Proceed immediately to Signal Development, below. Signal Development 1)   Dilute the 20X DAB Chromogen supplied with the kit in a sterile microcentrifuge tube just prior to use

IMMUNOCYTOCHEMICAL IDENTIFICATION OF PROLIFERATING CELLS IN VASCULAR TISSUE (ANTI-BrdU); Diaminobenzadine (DAB) brown detection

from Vector DAB substrate kit (Catalog No. SK-4100), four drops DAB, two drops H2O2 , and two drops of Nickel from kit, in 15ml tube (wrap the tube in foil to protect the substrate from light) . Incubate in the dark for 20-30 min., stop color reaction