Chemicon Millipore Osteogenesi

Osteogenesis Quantitation Kit

Chemicon (Millipore)

CHEMICON®'s Osteogenesis Quantitation Kit provides all the necessary reagents and a protocol to enable quantification of osteogenesis using a standard plate reader. This product is useful for studying the effects of growth factors, drugs and toxic agents on bone formation and for screening candidate osteogenic pharmaceuticals. Also, studies examining the intracellular signaling pathways regulating osteoblast differentiation can be facilitated by use of this kit.

The kit includes Alizarin Red Solution, a staining solution which can be used to visually detect the presence of mineralization in bone tissue. Also included are reagents for extraction of Alizarin Red from stained cells, and a protocol for performing quantitative analysis of the extracted Alizarin Red. Enough reagents are provided to perform 48 quantitation assays on 24 well plates.

For Research Use Only. Not for use in diagnostic procedures.

Note: Kit componenet 10X ARS Dilution Buffer: Part No. 2004810
One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent is provided. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use.

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Chromogenic

• Cell Based Assays
• Proliferation & Cell Growth Assays

• Alizarin Red S Stain Solution (1X): Part No. 2003999, One bottle containing 50 mL Alizarin Red S Solution. Store at 2-8°C.
• 10% Acetic Acid: Part No. 2004807, One bottle containing 20 mL of 10% Acetic Acid in deionized water. Store at 2-8°;C.
• 10% Ammonium Hydroxide: Part No. 2004809, One bottle containing 10 mL of 10% Ammonium Hydroxide in deionized water. Store at 2-8°C.
• 10X ARS Dilution Buffer: Part No. 2004810, One vial containing 5 mL of ARS (Alizarin Red Stain) Diluent. Add 5 mL of 80% Acetic Acid for a final volume of 10 mL before use. Store at 2-8°C.

• Phosphate-Buffered Saline (1X PBS)
• Fixative for Alizarin Red Staining
(e.g. 10% formaldehyde, or 4% paraformaldehyde, or 70% ethanol)
• Hemocytometer
• Microscope
• Plate reader capable of reading at 405 nm

All components should be stored at 2 - 8ºC until expiration date printed on label.