- 询价
- AB1582
- 2025年12月27日
- Western Blotting;Immunohistochemistry
- Sheep
- Guinea Pig;Rat;Rabbit
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- 详细信息
- 技术资料
- 应用范围：
Western Blotting;Immunohistochemistry
- 宿主：
Sheep
- 适应物种：
Guinea Pig;Rat;Rabbit
- 抗原来源：
0
- 库存：
大量
- 是否单克隆：
2
- 规格：
100 μL
Product Images
Sheep anti-Choline Acetyltransferase (Catalog Number AB1582) staining of rat brain stem motor neurons.
Sheep anti-Choline Acetyltransferase (Catalog Number AB1582) staining of guinea pig myenteric plexus, cell bodies and nerve fibers.
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| Gp, R, Rb | IH, WB | Sheep | Serum | Polyclonal Antibody |
Description:
Anti-Choline Acetyltransferase
Alternate Names:
ChAT
Specificity:
Choline Acetyltransferase (ChAT). Will stain cholinergic neurons in rat, guinea-pig and rabbit central and peripheral nervous systems.
Key Applications:
- Immunohistochemistry
- Western Blotting
Application Notes:
Immunohistochemistry: 1:1,000-1:2,000
Immunoblotting
Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1582
STAINING OF GUINEA PIG ILEUM
1) Pin the ileum out flat and tight.
2) Fix overnight at 4°C in modified Zamboni's (2% paraformaldehyde with 15% saturated picric acid in phosphate buffered saline).
3) Clear in DMSO, 100%, 3 times for 10 minutes each, rinse in PBS.
4) Dissect off ALL circular muscle.
5) Preincubate with 10% normal donkey serum.
6) Incubate in anti-ChAT (AB1582) diluted 1:1,000-1,2000 with 10% normal donkey serum over night at 4°C on a shaker.
7) Incubate with Donkey anti-Sheep IgG-Cy3 labeled secondary antibody (Catalog Number AP184C) at a dilution of 1:200 for 2 hours.
IMPORTANT NOTE: It is essential to remove ALL circular muscle.
Immunoblotting
Optimal working dilutions must be determined by end user.
IMMUNOHISTOCHEMISTRY PROTOCOL FOR AB1582
STAINING OF GUINEA PIG ILEUM
1) Pin the ileum out flat and tight.
2) Fix overnight at 4°C in modified Zamboni's (2% paraformaldehyde with 15% saturated picric acid in phosphate buffered saline).
3) Clear in DMSO, 100%, 3 times for 10 minutes each, rinse in PBS.
4) Dissect off ALL circular muscle.
5) Preincubate with 10% normal donkey serum.
6) Incubate in anti-ChAT (AB1582) diluted 1:1,000-1,2000 with 10% normal donkey serum over night at 4°C on a shaker.
7) Incubate with Donkey anti-Sheep IgG-Cy3 labeled secondary antibody (Catalog Number AP184C) at a dilution of 1:200 for 2 hours.
IMPORTANT NOTE: It is essential to remove ALL circular muscle.
Species Reactivity:
- Guinea Pig
- Rat
- Rabbit
Immunogen:
A 22 amino acid peptide from porcine ChAT, coupled to KLH. The sequence is H-GLFSSYRLPGHTQDTLVAQKSS-NH2.
Presentation:
Sheep serum. Lyophilized, no preservatives. Reconstitute with 100 μL of sterile distilled water. Centrifuge to remove any residue.
Storage Conditions:
Maintain at -20 to -70°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. Glycerol (1:1) can be added for additional stability.
Gene Symbol:
- CHAT
- ChAT
- CMS1A2
- CHOACTase
- CMS1A
- EC 2.3.1.6
Antibody Category:
Neuroscience
Antibody Sub-Category:
- Neurotransmitters & Receptors
- Neuronal & Glial Markers
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Chemicon (Millipore)
Format:
Serum
Host:
Sheep
Antibody Type:
Polyclonal Antibody
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
A 22 amino acid peptide from porcine ChAT, coupled to KLH. The sequence is H-GLFSSYRLPGHTQDTLVAQKSS-NH2. 详细描述见链接：http://www.millipore.com/catalogue/item/AB1582
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Anti-Choline Acetyltransferase; 100 μL
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