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文献和实验Biotin-4-fluorescein (B4F) is a convenient molecular probe for (strept)avidin and for unlabeled biotin in homogeneous fluorescence assays. The primary standard is a 16 μM working solution of d-biotin which is used to titrate an aliquot
Enzymatic Fluorescence and Biotin Labeling of Primers for PCR Sequencing
of nonisotopic detection with cycle sequencing offers several alternatives. The incorporation of the fluorescent label into the nascent DNA has been approached from several angles. At present, one may consider three sources of fluorescence (Fig. 1 ), irrespective
The biotin–avidin microplate assay is a sensitive method to measure methylation of biotinylated oligonucleotide substrates by DNA methyltransferases (MTases). The methylation reaction is carried out in solution using [methyl-3 H]-AdoMet
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