Anti-Choline Acetyltransferase; 500 μL

Anti-Choline Acetyltransferase

; 500 μL
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  • 询价
  • 2025年07月13日
  • Electron Microscopy;Western Blotting
  • Goat
  • Avian;Chicken;Guinea Pig
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    • 详细信息
    • 技术资料
    • 应用范围：

      Electron Microscopy;Western Blotting

    • 宿主：

      Goat

    • 适应物种：

      Avian;Chicken;Guinea Pig

    • 库存：

      大量

    • 抗原来源：

      0

    • 是否单克隆：

      2

    • 规格：

      500 μL

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    Goat anti-ChAT (Catalogue No. AB144P) staining of organotypic slice cultures of septum from 7-day-old rat tissue maintained in culture for 8 days.

    Coronal section of rat neocortex stained with catalog number AB144P showing the choline acetyltransferase positive cholinergic nerve fibers and terminals. Reference: D. Anandh, K Shobha and Dr. Bindu M Kutty, Department of Neurophysiology, National Institute of Mental Health and Neurosciences, Bangalore, India.

    Western Blot Analysis:
    Mouse brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-CHAT (1:1000 dilution of this lot).
    Proteins were visualized using a rabbit anti-goat secondary antibody conjugated to HRP and a chemiluminescence detection system.

    Arrow indicates protein CHAT (~68-70 kDa).

    Western Blot Analysis:
    Representative lot data.
    NIH/3T3 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-CHAT (1:1000 dilution).
    Proteins were visualized using a rabbit anti-goat secondary antibody conjugated to HRP and a chemiluminescence detection system.

    Arrow indicates protein ChAT (~68-70 kDa).

    Western Blot Analysis:
    Representative image from a previous lot.
    A431 (Lane1), C2C12 lysate (Lane2), HEK293 lysate (Lane 3), Hela lysate (Lane 4), HepG2 lysate (Lane 5), Human Placenta lysate (Lane 6), Huvec lysate (Lane 7), Jurkat lysate (Lane 8), L6 lysate (Lane 9), Mouse brain lysate (Lane 10), NIH/3T3 lysate (Lane 11), PC12 lysate (Lane 12), PC3 lysate (Lane 13), RAW264.7 lysate(Lane 14) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-ChAT (1:1000 dilution of a previous lot).
    Proteins were visualized using a rabbit anti-goat secondary antibody conjugated to HRP and a chemiluminescence detection system.

    Arrow indicates protein CHAT (~68-70 kDa).

    Species Reactivity Key Applications Host Format Antibody Type
    H, R, M, Mk, Op, Av, Ch, Gp, Zebrafish EM, IC, IH, WB Goat Affinity Purified Polyclonal Antibody
    Description:
    Anti-Choline Acetyltransferase
    Background Information:
    Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-choline acetyltransferase a useful specific marker.
    Alternate Names:
    • Choline acetylase
    • acetyl CoA:choline O-acetyltransferase
    • choline acetyltransferase
    Specificity:
    Choline acetyltransferase found in cholinergic neurons in brain and spinal cord.
    Key Applications:
    • Electron Microscopy
    • Immunocytochemistry
    • Immunohistochemistry
    • Western Blotting
    Application Notes:
    Immunohistochemistry:
    A previous lot of this antibody was used at 1:100.

    Optimal working dilutions and protocols must be determined by end user.
    Species Reactivity:
    • Human
    • Rat
    • Mouse
    • Monkey
    • Opossum
    • Avian
    • Chicken
    • Guinea Pig
    • Zebrafish
    Species Reactivity Note:
    Human and mouse. Expected to cross-react with Avian, chicken, guinea pig, monkey, rat, and opposum.
    Demonstrated to react in zebrafish in THE JOURNAL OF COMPARATIVE NEUROLOGY 474:75–107 (2004).
    Immunogen:
    Human placental enzyme.
    Molecular Weight:
    70/74 kDa
    Control:
    Human placenta lysates, rat forebrain/rostral hypothalamus.
    Presentation:
    Purified goat polyclonal in buffer containing 5 mg/mL BSA and 0.2% sodium azide.
    Storage Conditions:
    Maintain at -20°C in undiluted aliquots for up to 1 year from date of receipt. Avoid repeated freeze/thaw cycles.
    For short term storage (3-4 weeks) maintain at 2-8°C.
    Gene Symbol:
    • CHAT
    • ChAT
    • CHOACTase
    • CMS1A
    • CMS1A2
    Quality Assurance:
    Routinely evaluated by Western Blot on mouse brain lysates.

    Western Blot Analysis:
    1:1000 dilution of this lot detected CHAT on 10 μg of mouse brain lysates.
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    • Neurotransmitters & Receptors
    • Neuronal & Glial Markers
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Concentration:
    1 mg/mL
    Antibody Type:
    Polyclonal Antibody
    Format:
    Affinity Purified
    Host:
    Goat
    Product Overview:
    Catalyzes the reversible synthesis of acetylcholine (ACh) from acetyl CoA and choline at cholinergic synapses.
    Alternate Description:
    Choline Acetyltransferase Antibody
    Purification Method:
    Affinity Purfied

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    Human placental enzyme. 详细描述见链接：http://www.millipore.com/catalogue/item/AB144P

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