- ￥5820
- Sino Biological
- 北京
- 40607-V08B
- 2026年01月26日
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- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant Human coronavirus(HCoV-OC43)Spike Protein(S1+S2 ECD, His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
100.00 µg
Human coronavirus (HCoV-OC43) Spike Protein (S1+S2 ECD, His Tag): 产品信息
纯度> 85 % as determined by SDS-PAGE.
内毒素
< 1.0 EU per μg protein as determined by the LAL method.
生物活性
Testing in progress
蛋白构建
A DNA sequence encoding the human coronavirus (HCoV-OC43) spike protein (S1+S2 ECD) (AVR40344.1) (Met1-Pro1304) was expressed with a polyhistidine tag at the C-terminus.
表达宿主
Baculovirus-Insect Cells
种属
HCoV-OC43
预测 N 端
Phe 13
分子量
The recombinant human coronavirus (HCoV-OC43) spike protein (S1+S2 ECD, His Tag) consists of 1303 amino acids and predicts a molecular mass of 145.1 kDa.
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文献和实验该产品被引用文献
1, Yu, J; et al. Direct activation of the alternative complement pathway by SARS-CoV-2 spike proteins is blocked by factor D inhibition. Blood, PMID: 32877502
2, Liu, T; et al. Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates. Nature biomedical engineering, PMID: 33122853
3, Anderson, EM; et al. Seasonal human coronavirus antibodies are boosted upon SARS-CoV-2 infection but not associated with protection. medRxiv : the preprint server for health sciences, PMID: 33200143
4, Sakharkar, M; et al. Prolonged evolution of the human B cell response to SARS-CoV-2 infection. Science immunology, PMID: 33622975
5, Klausberger, M; et al. A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests. EBioMedicine, PMID: 33906067
6, Ravindran, R; et al. Immune response dynamics in COVID-19 patients to SARS-CoV-2 and other human coronaviruses. PloS one, PMID: 34242356
7, Jennewein, MF; et al. Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjects. Cell reports, PMID: 34237283
8, Natarajan, H; et al. Markers of Polyfunctional SARS-CoV-2 Antibodies in Convalescent Plasma. mBio, PMID: 33879585
9, Wratil, PR; et al. Evidence for increased SARS-CoV-2 susceptibility and COVID-19 severity related to pre-existing immunity to seasonal coronaviruses. Cell reports, PMID: 34932974
10, Lin, CY; et al. Pre-existing humoral immunity to human common cold coronaviruses negatively impacts the protective SARS-CoV-2 antibody response. Cell host & microbe, PMID: 34965382
11, Narowski, TM; et al. SARS-CoV-2 mRNA vaccine induces robust specific and cross-reactive IgG and unequal neutralizing antibodies in naive and previously infected people. Cell reports, PMID: 35090596 1
2, Lederer, K; et al. Germinal center responses to SARS-CoV-2 mRNA vaccines in healthy and immunocompromised individuals. Cell, PMID: 35202565 1
3, Pattinson, D; et al. A Novel Method to Reduce ELISA Serial Dilution Assay Workload Applied to SARS-CoV-2 and Seasonal HCoVs. Viruses, PMID: 35336970 1
4, Minervina, AA; et al. SARS-CoV-2 antigen exposure history shapes phenotypes and specificity of memory CD8 T cells. medRxiv : the preprint server for health sciences, PMID: 34341799 1
5, Lanz, TV; et al. Limited Neutralization of Omicron by Antibodies from the BNT162b2 Vaccination against SARS-CoV-2. Research square, PMID: 35441169 1
6, Minervina, AA; et al. SARS-CoV-2 antigen exposure history shapes phenotypes and specificity of memory CD8+ T cells. Nature immunology, PMID: 35383307 1
7, Sun, X; et al. Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2. Nature microbiology, PMID: 35773398 1
8, Anderson, EM; et al. SARS-CoV-2 infections elicit higher levels of original antigenic sin antibodies compared with SARS-CoV-2 mRNA vaccinations. Cell reports, PMID: 36261003 1
9, López-Muñoz, AD; et al. Cell Surface Nucleocapsid Protein Expression: A Betacoronavirus Immunomodulatory Strategy. bioRxiv : the preprint server for biology, PMID: 36993159
20, Ravindran, R; et al. Dynamics of temporal immune responses in nonhuman primates and humans immunized with COVID-19 vaccines. PloS one, PMID: 37856429
21, Dos Santos Alves, RP; et al. Human coronavirus OC43-elicited CD4+ T cells protect against SARS-CoV-2 in HLA transgenic mice. Nature communications, PMID: 38278784 2
2, Sibille, G; et al. The Novel A-Type Proanthocyanidin-Rich Phytocomplex SP4™ Acts as a Broad-Spectrum Antiviral Agent against Human Respiratory Viruses. International journal of molecular sciences, PMID: 39000477 2
3, Li, Z; et al. Affinity-independent memory B cell origin of the early antibody-secreting cell response in naive individuals upon SARS-CoV-2 vaccination. Immunity, PMID: 39168129 2
4, Verma, SK; et al. Influence of Th1 versus Th2 immune bias on viral, pathological, and immunological dynamics in SARS-CoV-2 variant-infected human ACE2 knock-in mice. EBioMedicine, PMID: 39353281 2
5, Li, S; et al. Dual-Locking the SARS-CoV-2 Spike Trimer: An Amphipathic Molecular "Bolt" Stabilizes Conserved Druggable Interfaces for Coronavirus Inhibition. Advanced science (Weinheim, Baden-Wurttemberg, Germany), PMID: 40285637 2
6, Bean, DJ; et al. Endemic coronavirus infection is associated with SARS-CoV-2 Fc receptor-binding antibodies. Journal of virology, PMID: 40387363
2, Liu, T; et al. Quantification of antibody avidities and accurate detection of SARS-CoV-2 antibodies in serum and saliva on plasmonic substrates. Nature biomedical engineering, PMID: 33122853
3, Anderson, EM; et al. Seasonal human coronavirus antibodies are boosted upon SARS-CoV-2 infection but not associated with protection. medRxiv : the preprint server for health sciences, PMID: 33200143
4, Sakharkar, M; et al. Prolonged evolution of the human B cell response to SARS-CoV-2 infection. Science immunology, PMID: 33622975
5, Klausberger, M; et al. A comprehensive antigen production and characterisation study for easy-to-implement, specific and quantitative SARS-CoV-2 serotests. EBioMedicine, PMID: 33906067
6, Ravindran, R; et al. Immune response dynamics in COVID-19 patients to SARS-CoV-2 and other human coronaviruses. PloS one, PMID: 34242356
7, Jennewein, MF; et al. Isolation and characterization of cross-neutralizing coronavirus antibodies from COVID-19+ subjects. Cell reports, PMID: 34237283
8, Natarajan, H; et al. Markers of Polyfunctional SARS-CoV-2 Antibodies in Convalescent Plasma. mBio, PMID: 33879585
9, Wratil, PR; et al. Evidence for increased SARS-CoV-2 susceptibility and COVID-19 severity related to pre-existing immunity to seasonal coronaviruses. Cell reports, PMID: 34932974
10, Lin, CY; et al. Pre-existing humoral immunity to human common cold coronaviruses negatively impacts the protective SARS-CoV-2 antibody response. Cell host & microbe, PMID: 34965382
11, Narowski, TM; et al. SARS-CoV-2 mRNA vaccine induces robust specific and cross-reactive IgG and unequal neutralizing antibodies in naive and previously infected people. Cell reports, PMID: 35090596 1
2, Lederer, K; et al. Germinal center responses to SARS-CoV-2 mRNA vaccines in healthy and immunocompromised individuals. Cell, PMID: 35202565 1
3, Pattinson, D; et al. A Novel Method to Reduce ELISA Serial Dilution Assay Workload Applied to SARS-CoV-2 and Seasonal HCoVs. Viruses, PMID: 35336970 1
4, Minervina, AA; et al. SARS-CoV-2 antigen exposure history shapes phenotypes and specificity of memory CD8 T cells. medRxiv : the preprint server for health sciences, PMID: 34341799 1
5, Lanz, TV; et al. Limited Neutralization of Omicron by Antibodies from the BNT162b2 Vaccination against SARS-CoV-2. Research square, PMID: 35441169 1
6, Minervina, AA; et al. SARS-CoV-2 antigen exposure history shapes phenotypes and specificity of memory CD8+ T cells. Nature immunology, PMID: 35383307 1
7, Sun, X; et al. Neutralization mechanism of a human antibody with pan-coronavirus reactivity including SARS-CoV-2. Nature microbiology, PMID: 35773398 1
8, Anderson, EM; et al. SARS-CoV-2 infections elicit higher levels of original antigenic sin antibodies compared with SARS-CoV-2 mRNA vaccinations. Cell reports, PMID: 36261003 1
9, López-Muñoz, AD; et al. Cell Surface Nucleocapsid Protein Expression: A Betacoronavirus Immunomodulatory Strategy. bioRxiv : the preprint server for biology, PMID: 36993159
20, Ravindran, R; et al. Dynamics of temporal immune responses in nonhuman primates and humans immunized with COVID-19 vaccines. PloS one, PMID: 37856429
21, Dos Santos Alves, RP; et al. Human coronavirus OC43-elicited CD4+ T cells protect against SARS-CoV-2 in HLA transgenic mice. Nature communications, PMID: 38278784 2
2, Sibille, G; et al. The Novel A-Type Proanthocyanidin-Rich Phytocomplex SP4™ Acts as a Broad-Spectrum Antiviral Agent against Human Respiratory Viruses. International journal of molecular sciences, PMID: 39000477 2
3, Li, Z; et al. Affinity-independent memory B cell origin of the early antibody-secreting cell response in naive individuals upon SARS-CoV-2 vaccination. Immunity, PMID: 39168129 2
4, Verma, SK; et al. Influence of Th1 versus Th2 immune bias on viral, pathological, and immunological dynamics in SARS-CoV-2 variant-infected human ACE2 knock-in mice. EBioMedicine, PMID: 39353281 2
5, Li, S; et al. Dual-Locking the SARS-CoV-2 Spike Trimer: An Amphipathic Molecular "Bolt" Stabilizes Conserved Druggable Interfaces for Coronavirus Inhibition. Advanced science (Weinheim, Baden-Wurttemberg, Germany), PMID: 40285637 2
6, Bean, DJ; et al. Endemic coronavirus infection is associated with SARS-CoV-2 Fc receptor-binding antibodies. Journal of virology, PMID: 40387363
相关实验
Titration of Human Coronaviruses, HCoV-229E and HCoV-OC43, by an Indirect Immunoperoxidase Assay
229E and OC43 of human coronavirus (HCoV). An alternative indirect immunoperoxidase assay (IPA) is herein described for the detection and titration of these viruses. Susceptible cells are inoculated with serial logarithmic dilutions of samples in a 96
HIS 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的 HIS 标签蛋白。该问题主要可以分为以下两个方面： 1、HIS 标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螫合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲
蛋白标签（protein tag）是指利用DNA体外重组技术，与目的蛋白一起融合表达的一种多肽或者蛋白，以便于目的蛋白的表达、检测、示踪和纯化等。随着技术的不断发展，研究人员相继开发出了具有各种不同功能的蛋白标签。 一、 新型蛋白标签 蛋白标签应用极为广泛，但仍有两个问题不容忽视：（1）蛋白标签以DNA形式编码，它需要转录并翻译成蛋白后才能发挥作用，而这种作用方式本身就是一种缺陷。例如，常用的荧光蛋白标签，在显微镜下观察时，其显像不如人工合成的荧光基团好，但是这种人工合成的荧
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文献支持
重组Human coronavirus(HCoV-OC43)Spike 蛋白(S1+S2 ECD, His 标签)
￥5820
