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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件:
2-8°C or -15 to -30°C
- 保质期:
N/A
- 英文名:
Stripping Buffer
- 库存:
部分现货库存
- 供应商:
青木生物技术(武汉)有限公司
- 规格:
50 ml/125 ml/500 ml
| 规格: | 50 ml | 产品价格: | ¥690.0 |
|---|---|---|---|
| 规格: | 125 ml | 产品价格: | ¥943.0 |
| 规格: | 500 ml | 产品价格: | ¥1817.0 |
After stripping the membrane can be used for repeated detection (reprobing) with antibodies.
Stripping Buffer does not contain mercapto-ethanol nor DTT.
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- 询问日期
文献和实验1) After ECL development, wash membrane once for 10min with PBST. 2) Incubate the membrane in stripping buffer (see below) in a heat-sealed plastic bag for 30min at 50oC with occasional mixing. For low-abundance antigens, the strip
Probing and Stripping of Western Blot
).13. If the membrane will be reprobed in the next day or two without stripping, wash several times in TBST and store at 4 degrees in TBST.10X TBS 1 liter200 mM 24.2 g TrispH7.6 Tris1.37 M
Stripping for reprobing western blots
minutes. (2) Discard buffer. (3) 5-10 minutes fresh stripping buffer. (4) Discard buffer. (5) 10 minutes PBS (6) 10 minutes PBS (7) 5 minutes TBST (8) 5 minutes TBST Ready










