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- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 保质期：
请询
- 英文名：
Candor Bioscience：AP-Defender
- 库存：
现货库存
- 供应商：
青木生物技术（武汉）有限公司
- 规格：
50 ml/125 ml/500 ml
| 规格： | 50 ml | 产品价格： | 询价 |
|---|---|---|---|
| 规格： | 125 ml | 产品价格： | 询价 |
| 规格： | 500 ml | 产品价格： | 询价 |
Diluent for long-term storage of alkaline phosphatase conjugates
AP-Defender is a long-term stabilizer for alkaline phosphatase (AP) coupled to antibodies or Neutravidin/Streptavidin. AP-Defender can give peroxidase conjugates the crucial long-term stability over several years even when stored in low concentrated ready-to-use dilutions. AP-Defender can be used directly as an assay buffer for incubating the AP-conjugates.
Typical ready-to-use concentrations for detection are between 100-1000 ng/mL.
Stability data of one AP-conjugate cannot be directly transferred to other conjugates. Each conjugatemust be tested for its individual shelf-life in AP-Defender.
If AP-Defender is used in immunodiagnostic kits, the shelf life must be validated according to the applicable regulatory requirements for diagnostics.
AP-Defender contains components that may interfere with commonly used conjugation methods, such as techniques targeting primary amines and sulfhydryl groups. Suitability for any given conjugation method must be tested in advance. We recommend diluting biomolecules in AP-Defender only after conjugation.
Suitability of AP-Defender for a specific assay must be tested by the user.
Please note that high protein concentrations and/or microbial contamination may reduce the effectiveness of the preservative. If antibodies/conjugates are added for storage in a non-sterile manner and microbial contamination is suspected, higher-than-usual preservative concentrations may be required.
图：将250ng/ml Goat-anti-Rabbit IgG-AP存放在不同的AP-Defender溶液中，加速稳定性实验表明，AP-Defender(CR335)可以使AP偶联物在 40℃稳定97天，性能远优于市场同类产品。
For general laboratory use
AP-Defender is also available as a 5x concentrate: AP-Defender (5x) (catalog no. 336).
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CANDOR BIOSCIENCE: 关于ELISA板稳定性的技术探讨与比较
稳定剂，例如CANDOR的Antibody Stabilizer、HRP-Protector™和AP-Protector®，可以大大提高对照品和检测抗体-结合物的稳定性。本文以ELISAs为例，重点讨论固相稳定、表面包被捕获分子的活性维持的机理和解决方案。此外，本文概述的原理和解决方案也可以应用于其他包被表面，如聚苯乙烯或玻璃表面的实验应用，如侧向层析、亲和柱、基于微球的分析、免疫PCR或蛋白质分析等。 包被的捕获分子如抗体，或在抗原下降分析中分离的病毒蛋白，是可靠免疫分析的核心组成
7.6,高背景或 AP 存在时推荐使用 C0905 荧光抗体专用稀释液 10ml 50 pH 7.2,不能加入血清 C0906 HRP稳定剂 10ml/100ml 50/350 pH7.2，无蛋白成分 *-所有稀释液在4℃保存抗体时，能稳定
酶、α-1抗胰蛋白酶或纤维蛋白原等高度丰富的血清蛋白都会干扰免疫分析。此外，标记检测抗体或示踪剂分析物是免疫分析中的常见做法。标签可以是酶（如碱性磷酸酶（AP）或辣根过氧化物酶（HRP））、荧光染料、放射性同位素、亲和力标签，如生物素，或者DNA（immuno-PCR中采用）。这些标签也会改变标记对象的属性，并带来新的干扰。 亲和力鉴别法消除干扰 以上提到的这些干扰有一个共同点：它们是由中低亲和力结合问题引起的。相反，一个好的抗体与其表位之间的特异性相互作用具有很高的亲和力。然而，即使足够
