- 询价
- APT110
- 2026年01月11日
- Flow Cytometry
- 0
- Vertebrates
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Flow Cytometry
- 宿主：
0
- 抗原来源：
0
- 库存：
大量
- 适应物种：
Vertebrates
- 是否单克隆：
0
- 规格：
50 assays
- ApoDIRECT
- Chemicon (Millipore)
- Apoptosis Assays
- Apoptosis Assays
- The following components are included with the APO-DIRECT™ Kit. Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 50 cell suspensions including control cells of approximately 1 x 106 cells per suspension for flow cytometry analysis. The control cells have been fixed as described (under the heading cell fixation procedure) and are in 70% (v/v) ethanol.
- Positive Control Cells,Brown cap
- Negative Control Cells, Natural cap
- Wash Buffer, Blue cap
- Reaction Buffer, Green cap
- TdT Enzyme, Yellow cap
- F-dUTP, Orange cap
- Rinsing Buffer, Red cap
- PI/RNase Staining Buffer, Amber bottle
1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.
2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid areas of contact immediately. See Material Safety Data Sheets.
3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.
2. Distilled Water
3. 1% (w/v) paraformaldehyde (methanol free) in PBS
4. 70% (v/v) ethanol
5. 37oC Water Bath
6. Ice Bucket
7. 12 x 75 mm flow cytometry test tubes
8. Micro-pipettor
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
详细描述见链接：http://www.millipore.com/catalogue/item/APT110
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文献和实验Detection of apoptotic process in situ using immunocytochemical and TUNEL assays
, however, can not provide information regarding the histological localization at single cell level. This can be done by immunocytochemical method (A) or by enzymatic in situ labeling of apoptosis-induced DNA strand breaks (TUNEL) (B). Apoptosis was observed
´. Wash 3 x 5´ in water. Wash 1 x 5´ in 1x PBS (Phosphate-Buffered Saline). •Shake off/wipe off excess PBS and circle all sections with ImmunoEdge or PAP pen. •Block 10 minutes in Equilibration Buffer at room temperature (50 μl/section). •Add 50 μl
to the collection tube. Close the tube and vortex briefly. Make sure the ground seeds is covered by the Extraction Solution. b. Incubate at 56°C for 10-20 minutes. c. Incubate at 95°C for 5 minutes. d. Add 50 μl PS3 buffer
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