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Silver Membrane FiltersUse for air monitoring of carbon black, coal tar products, coke oven emissions, and silica. Referenced by NIOSH for collecting airborne contaminants Ideal for crystalline silica analysis by X-ray diffraction techniques. |
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/AG4502550
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文献和实验Dithiothreitol (DTT) 5 µg/ml* 0.1% silver nitrate* 3% sodium carbonate 37% formaldehyde 2.3M citric acid (* these solutions must be prepared freshly) Staining procedure All volumes are 300 ml for 1.5 x 160 x 160 mm gel (biorad
: 2.5 mM MgCl2 3 mM KCl 0.5% Triton X-100 in PBS Add protease inhibitors PMSF: 1 mL per 100 mL buffer Use fresh PMSF: dissolve 0.035g per 1mL 100%EtOH
Materials Protein gel from Exercise 4.2 45% (v/v) Methanol + 12% (w/v) acetic acid 5% (v/v) Methanol + 7% (w/v) acetic acid 10% Glutaraldehyde 0.01M Dithiothreitol Silver nitrate solution
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