Anti-Choline Acetyltransferase

Anti-Choline Acetyltransferase (ChAT)

Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.

• Choline acetylase
• acetyl CoA:choline O-acetyltransferase
• choline acetyltransferase

Cholinergic neurons in brain and central nervous systems.

• Immunohistochemistry
• Immunohistochemistry (Paraffin)
• Immunoprecipitation
• Western Blotting

Immunoprecipitation:
1:200 dilution of a previous lot immunoprecipitated 68 kDa active ChAT from brain and placenta. Also precipitates a 27 kDa protein that is co-expressed with ChAT (but lacking ChAT activity) in some cholinergic neurons of the central nervous system of Humans and possibly other mammals.

Optimal working dilutions and protocls must be determined by end user.

• Human
• Rat
• Mouse
• Bat
• Feline
• Monkey

Human, Mouse, Rat, Monkey, Bat, Feline. Reactivity with other species has not been confirmed with IHC.

Human placental enzyme.

Cerebral cortex tissue.

Rabbit polyclonal antisera, liquid, no preservatives.

Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

• CHAT
• ChAT
• CHOACTase
• CMS1A
• CMS1A2

Routinely evaluated by immunohistochemistry (paraffin) on normal cerebral cortex tissue.

Immunohistochemistry (Paraffin) Analysis:
ChAT (cat. # AB143) staining on normal cerebral cortex. Tissue treated with no antigen retrieval. The antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern on the cell body, dendrites and axons of neurons (brown).

Neuroscience

• Neurotransmitters & Receptors
• Neuronal & Glial Markers

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Chemicon (Millipore)

Serum

Rabbit

Variable

Polyclonal Antibody