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Anti-Choline Acetyltransferase

; 50 μL
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  • 询价
  • 2025年12月25日
  • Immunoprecipitation;Immunohistochemistry
  • Rabbit
  • Mouse;Monkey;Rat;Bat
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      Immunoprecipitation;Immunohistochemistry

    • 宿主：

      Rabbit

    • 抗原来源：

      0

    • 适应物种：

      Mouse;Monkey;Rat;Bat

    • 库存：

      大量

    • 是否单克隆：

      2

    • 规格：

      50 μL

    Product Images

    Immunohistochemistry (Paraffin) Analysis:
    Representative image from a previous lot.
    ChAT (cat. # AB143) staining on normal cerebral cortex. ...
    Species Reactivity Key Applications Host Format Antibody Type
    H, R, M, Bat, Fe, Mk IH, IH(P), IP, WB Rabbit Serum Polyclonal Antibody
    Description:
    Anti-Choline Acetyltransferase (ChAT)
    Background Information:
    Acetylcholine (ACh) is a common neurotransmitter for motoneurons, preganglionic autonomic neurons, postganglionic parasympathetic neurons, a variety of brain regions and some emerging neuron-like stem cells. The metabolism of Ach is relatively simple, involving only two enzymes: choline acetyltransferase (ChAT) for synthesis and acetylcholinesterase (AChE) for degradation. Further, acetylcholine has little function in neurons other than neurotransmission and seems to be neuron specific. It seems that only cholinergic neurons have significant amounts of ChAT making anti-cholinacetyltransferase a useful specific marker.
    Alternate Names:
    • Choline acetylase
    • acetyl CoA:choline O-acetyltransferase
    • choline acetyltransferase
    Specificity:
    Cholinergic neurons in brain and central nervous systems.
    Key Applications:
    • Immunohistochemistry
    • Immunohistochemistry (Paraffin)
    • Immunoprecipitation
    • Western Blotting
    Application Notes:
    Immunoprecipitation:
    1:200 dilution of a previous lot immunoprecipitated 68 kDa active ChAT from brain and placenta. Also precipitates a 27 kDa protein that is co-expressed with ChAT (but lacking ChAT activity) in some cholinergic neurons of the central nervous system of Humans and possibly other mammals.

    Optimal working dilutions and protocls must be determined by end user.
    Species Reactivity:
    • Human
    • Rat
    • Mouse
    • Bat
    • Feline
    • Monkey
    Species Reactivity Note:
    Human, Mouse, Rat, Monkey, Bat, Feline. Reactivity with other species has not been confirmed with IHC.
    Immunogen:
    Human placental enzyme.
    Control:
    Cerebral cortex tissue.
    Presentation:
    Rabbit polyclonal antisera, liquid, no preservatives.
    Storage Conditions:
    Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
    Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    Gene Symbol:
    • CHAT
    • ChAT
    • CHOACTase
    • CMS1A
    • CMS1A2
    Quality Assurance:
    Routinely evaluated by immunohistochemistry (paraffin) on normal cerebral cortex tissue.

    Immunohistochemistry (Paraffin) Analysis:
    ChAT (cat. # AB143) staining on normal cerebral cortex. Tissue treated with no antigen retrieval. The antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern on the cell body, dendrites and axons of neurons (brown).
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    • Neurotransmitters & Receptors
    • Neuronal & Glial Markers
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    Serum
    Host:
    Rabbit
    Concentration:
    Variable
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    Human placental enzyme. 详细描述见链接：http://www.millipore.com/catalogue/item/AB143

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