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- APT115
- 2026年01月11日
- Flow Cytometry
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Flow Cytometry
- 宿主：
0
- 适应物种：
All
- 抗原来源：
0
- 库存：
大量
- 是否单克隆：
0
- 规格：
50 assays
Product Images
Br-dUrd Incorporation & Labeling
- The APO-BRDU™ Kit is shipped in one container and consists of two packages. One package is shipped at ambient temperature and should be stored at 2-8°C upon arrival. The other package is styrofoam containing frozen ice packs and the reagent contents should be stored at -20°C upon arrival. Note: Upon arrival store the reagents at the appropriate temperatures.
- Reagent bottles have color coded caps to aid in their identification. Sufficient reagents are provided to process 60 cell suspensions including 5 mL positive and 5 mL negative control cell suspensions of approximately 1 x 106 cells per mL in 70% (v/v) ethanol. The control cells are derived from a human lymphoma cell line and have been fixed as described on page 6.
- Positive Control Cells, Brown cap
- Negative Control Cells, White cap
- Wash Buffer, Blue cap
- Reaction Buffer, Green cap
- TdT Enzyme, Yellow cap
- Br-dUTP, Violet cap
- Rinsing Buffer, Red cap
- Fluorescein~PRB-1 mAb, Orange cap
- PI/RNase Staining Buffer, Amber bottle
1. The components of this kit are for Research Use only and are not intended for diagnostic procedures.
2. Positive and negative control cells contain 70% (v/v) ethanol as a preservative; wash and reaction buffer contain sodium cacodylate (dimethylarsinic) as a buffer; rinsing and PI/RNase staining buffer contain 0.05% (w/v) sodium azide as a preservative. These materials are harmful if swallowed; avoid skin contact, wash immediately with water. See Material Safety Data Sheets.
3. TdT Enzyme will not freeze at -20oC, because it is in 50% (v/v) glycerol solution. Upon warming the TdT enzyme solution, centrifuge the tube for 30 seconds to force all the liquid to the bottom of the tube.
2. Distilled Water
3. 1% (w/v) paraformaldehyde (methanol free) in PBS
4. 70% (v/v) ethanol
5. 37°C Water Bath
6. Ice Bucket
7. 12 x 75 mm flow cytometry test tubes
8. Micro-pipettor
Product Family Information
BrdUMillipore’s Anti-BrdU antibody demonstrates specificity against BrdU. See below for data, references and related products for BrdU. All Millipore antibodies are based on the expertise of Upstate & Chemicon. |
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
详细描述见链接：http://www.millipore.com/catalogue/item/APT115
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文献和实验Detection of Infectious Rotaviruses by Flow Cytometry
(2 ). Since an extremely high number of virus particles are present in the feces during the acute gastroenteritis, methods based on electron microscopy, passive particle agglutination tests, or enzyme-linked immunosorbent assays are readily employed for clinical diagnosis
Flow Cytometry Assays in Primary Immunodeficiency Diseases
mutations lead to abnormalities that can be detected in circulating peripheral blood cells of suspected patients by flow cytometry and the appropriate combinations of reagents and in vitro manipulations. The flow cytometry procedures that have been developed
A SENSITIVE METHOD FOR DETECTION OF APOPTOSIS BY SINGLE LASER FLOW CYTOMETRY
immunofluorescence by single laser flow cytometry. J Immunol Meth 170: 145-157, 1994. Staining for detection of apoptosis 1 x 106 PBSAz-washed cells from a single cell suspension are pelleted in a 12 X 75mm culture tube. The pellet is resuspended
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