Anti-Neurofilament H (200 kDa)

Anti-Neurofilament H (200 kDa), C-terminus

This antibody replaces AB1982.

Neurofilaments are a type of intermediate filament that serve as major elements of the cytoskeleton supporting the axon cytoplasm. They are the most abundant fibrillar components of the axon, being on average 3-10 times more frequent than axonal microtubules. Neurofilaments (10nm in dia.) are built from three intertwined protofibrils which are themselves composed of two tetrameric protofilament complexs of monomeric proteins. The neurofilament triplet proteins (68/70, 160, and 200 kDa) occur in both the central and peripheral nervous system and are usually neuron specific. The 68/70 kDa NF-L protein can self-assemble into a filamentous structure, however the 160 kDa NF-M and 200 kDa NF-H proteins require the presence of the 68/70 kDa NF-L protein to co-assemble. Neuromas, ganglioneuromas, gangliogliomas, ganglioneuroblastomas and neuroblastomas stain positively for neurofilaments. Although typically restricted to neurons, neurofilaments have been detected in paragangliomas and adrenal and extra-adrenal pheochromocytomas. Carcinoids, neuroendocrine carcinomas of the skin, and oat cell carcinomas of the lung also express neurofilaments. For more neurofilament information see Nervous System Cell Type Specific Marker chart online under the CHEMICON Technical Support section.

Strong and specific staining for NF-H with no cross-reactivity for NF-M or NF-L. Reacts with both the phosphorylated and dephosphorylated forms of neurofilament 200 kDa. Stains neurons of the central and peripheral nervous system, including new processes in all organs. Preferentially stains nerve processes.

• Immunohistochemistry
• Western Blotting

Immunoblotting

Immunohistochemistry: 1:200

Optimal working dilutions must be determined by end user.

• Bovine
• Human
• Mouse
• Pig
• Rat

Recombinant fusion protein containing the extreme C-terminus of rat NF-H, prepared from E. coli inclusion bodies by dissolution in urea followed by DEAE-cellulose ion exchange chromatography. Immunogen does not include the lysine-serine-proline

C-terminus

200 kDa

Brain, Peripheral Nerve

Neat rabbit antisera, no preservatives.

Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

• NEFH
• NFH
• NF-H
• KIAA0845

Neuroscience

• Neurofilament & Neuron Metabolism
• Neuronal & Glial Markers

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Chemicon (Millipore)

Serum

Rabbit

Variable

Polyclonal Antibody