- 询价
- 07-1490
- 2025年07月11日
- Western Blotting;Immunohistochemistry (Paraffin)
- Rabbit
- Mouse;Human;Mammals
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Western Blotting;Immunohistochemistry (Paraffin)
- 宿主：
Rabbit
- 适应物种：
Mouse;Human;Mammals
- 库存：
大量
- 抗原来源：
0
- 是否单克隆：
2
- 规格：
100 μL
Immunohistochemistry (Paraffin): Representative image from a previous lot. Calcineurin representative staining pattern/morphology in human vascular tissue. Tissue was pretreated with citrate pH 6, antigen retrieval. A previous lot of this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.
Western Blot Analysis:
PC3 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-CALCINEURIN (1:500 dilution of this lot).
Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates protein PP2B B (~19 kDa).
Western Blot Analysis:
Representative image from a previous lot.
RAW 264 lysate (Lane1), HepG2 lysate (Lane 6), HeLa lysate (Lane 7) and PC3 lysate (Lane 8) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-Calcineurin (1:500 dilution of a previous lot).
Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates protein Calcineurin (~19 kDa).
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, H, Ma | WB, IH(P), IP | Rabbit | Affinity Purified | Polyclonal Antibody |
- Western Blotting
- Immunohistochemistry (Paraffin)
- Immunoprecipitation
Immunoprecipitation: 3 μL per reaction of a previous lot of this antibody was used in Immunoprecipitation.
Optimal working dilutions must be determined by end user
- Mouse
- Human
- Mammals
Broad reactivity with mammal species is expected.
Handling Recommendations: Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
- PPP3CA
- CCN1
- PPP2B
- CNA1
- CAN
- CALNA1
- CALN
- CALNA
- EC 3.1.3.16
Western Blot Analysis:
1:500 dilution of this lot detected PP2B B on 10 μg of PC3 lysates.
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Purified bovine brain calcineurin. 详细描述见链接：http://www.millipore.com/catalogue/item/07-1490
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文献和实验孔，在第一、第二孔中分别加标准品 100μl ，然后在第一、第二孔中加标准品稀释液 50μl ，混匀；然后从第一孔、第二孔中各取 100μl 分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液 50μl ，混匀；然后在第三孔和第四孔中先各取 50μl 弃掉，再各取 50μl 分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液 50ul ，混匀；混匀后从第五、第六孔中各取 50μl 分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液 50μl ，混匀后从第七、第八孔中
附表1：转换因子Z(μl/mg)数值表（无菌水） 温度 气压（KPa） 1KPa=10hPa （℃） 80 85 90 95 100 101.3 105 15.00 1.0017
采用回收的聚丙烯（这种情况下，我们只能说它的主要成分是聚丙烯），这类吸头一般柔软性很差，吸头壁较厚。而柔软性差的吸头容易导致吸头与移液器的密封一致性差；吸头壁厚则容易使液体残留，且排液不灵活。 二、 吸头的制造和生产工艺 除了吸头原材料之外，大多数吸头在制造的过程中还会加入显色材料和少量的添加剂，比较常见的有： 1. 显色材料。市场上俗称的蓝色吸头（1000μL）和黄色吸头（200μL），就是在聚丙烯中加入了相应的显色材料。显色材料需要用高品质的色母粒，而非低廉的工业
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