- 询价
- 07-838
- 2025年07月15日
- Western Blotting
- Rabbit
- Human;Mouse
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Western Blotting
- 宿主：
Rabbit
- 抗原来源：
0
- 库存：
大量
- 适应物种：
Human;Mouse
- 是否单克隆：
2
- 规格：
100 μL
Synthetic peptide corresponding to the amino acid region encompassing the human phospho-IR (Tyr972 (960)) in the juxtamembrane domain. 详细描述见链接：http://www.millipore.com/catalogue/item/07-838
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文献和实验separating buffer with 13.0 mL of water, 10.5 mL of acrylamide/bis solution, 100 μL of ammonium persulfate solution, and 20 μL of TEMED. Pour the separating gel,and overlay with water-saturated isobutanol. 3. Pour off the isobutanol and rinse
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
amounts of lysate(usually 0.1–10 μL in a total volume of 100 μL of water) to1 mL of Coomassie (Bradford) reagent, vortex, and incubateat room temperature for 10 min. Vortex the solutions again,then measure the absorbance using a spectrophotometer atl max
Using Phospho‐Motif Antibodies to Determine Kinase Substrates
Figure 18.20.1 The specificity of phospho‐motif antibodies. HeLa cells were serum‐starved for 24 hr prior to being stimulated with 50 ng/ml IGF‐1 or 100 nM PMA for 10 min. Lysates were immunoblotted with anti‐Akt/PKB phospho‐motif (left panel
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