- 询价
- AB144P-200UL
- 2025年12月25日
- Western Blotting;Immunocytochemistry
- Goat
- Avian;Chicken;Guinea Pig
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- 文献和实验
- 技术资料
- 应用范围：
Western Blotting;Immunocytochemistry
- 宿主：
Goat
- 库存：
大量
- 适应物种：
Avian;Chicken;Guinea Pig
- 抗原来源：
0
- 是否单克隆：
2
- 规格：
200 μL
Goat anti-ChAT (Catalog Number AB144P-200UL) staining of rat hypothalamus. Photograph courtesy of Dr. Robert Sloviter, University of Arizona.
Western Blot Analysis:
Mouse brain lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-CHAT (1:1000 dilution of this lot).
Proteins were visualized using a rabbit anti-goat secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates protein CHAT (~68-70 kDa).
Western Blot Analysis:
Representative lot data.
NIH/3T3 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-CHAT (1:1000 dilution).
Proteins were visualized using a rabbit anti-goat secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates protein ChAT (~68-70 kDa).
Western Blot Analysis:
Representative image from a previous lot.
A431 (Lane1), C2C12 lysate (Lane2), HEK293 lysate (Lane 3), Hela lysate (Lane 4), HepG2 lysate (Lane 5), Human Placenta lysate (Lane 6), Huvec lysate (Lane 7), Jurkat lysate (Lane 8), L6 lysate (Lane 9), Mouse brain lysate (Lane 10), NIH/3T3 lysate (Lane 11), PC12 lysate (Lane 12), PC3 lysate (Lane 13), RAW264.7 lysate(Lane 14) were resolved by electrophoresis, transferred to PVDF membrane and probed with anti-ChAT (1:1000 dilution of a previous lot).
Proteins were visualized using a rabbit anti-goat secondary antibody conjugated to HRP and a chemiluminescence detection system.
Arrow indicates protein CHAT (~68-70 kDa).
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| M, R, H, Ch, Av, Gp, Op | IC, IH, WB | Goat | Affinity Purified | Polyclonal Antibody |
- Choline acetylase
- acetyl CoA:choline O-acetyltransferase
- choline acetyltransferase
- Immunocytochemistry
- Immunohistochemistry
- Western Blotting
A previous lot of this antibody was used in immunohistochemistry at 1:100 dilution. See attached protocol.
Optimal working dilutions must be determined by end user.
- Mouse
- Rat
- Human
- Chicken
- Avian
- Guinea Pig
- Opossum
For short term storage (3-4 weeks) maintain at +2-8°C.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
- CHAT
- ChAT
- CHOACTase
- CMS1A
- CMS1A2
Western Blot Analysis:
1:1000 dilution of this antibody detected ChAT on 10 µg of NIH/3T3 lysates.
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
Human placental enzyme. 详细描述见链接：http://www.millipore.com/catalogue/item/AB144P-200UL
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血液基因组 DNA提取试剂盒操作指南（DP348）——200μl 以内抗凝全血
以下操作按照天根产品 DP348 血液基因组 DNA 提取试剂盒的说明书进行，所有反应现象仅适用于该产品及本文所标明的样本量。如使用其它操作流程或产品，样本量不同可能现象与本文有所差异。实验准备：1. 抗凝血 （200 μl以内抗凝全血，不足200 μl可加缓冲液GS补足体积至200 μl）2. 移液器及配套无菌枪头（200 μl ，1ml）3. 无水乙醇4. 涡旋振荡器，金属浴/水浴，台式离心机备注：本实验以人血为例，如提取哺乳动物全血可以用此流程，如果禽类、鸟类、两栖类或更低级生物的抗凝
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