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NovaSeptum® Sterile Sampling SystemsSterile, closed, disposable systems for sampling fluids from sterile and aseptic processes |
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文献和实验A general protocol for staining cell for cytometry analysis
General Annexin V Staining Procedure Solutions 1.10X Binding Buffer (Cat. No. 66121A):0.1 M HEPES, pH 7.4; 1.4 M NaCl; 25 mM CaCl 2 . Dilute to 1× prior to use. 2. Propidium Iodide (PI). Prepare a 50 µg/ml stock solution of PI in 1× PBS buffer
Single tube confirmation PCR protocol
50 µl total volume *10 x Taq buffer contains: 100 mM Tris-HCl (pH 8.4), 500 mM KCl, 15 mM MgCl2. *The final concentrations are shown in parentheses. *The Taq Polymerase should be added last and PCR mixture should be kept on ice
A general protocol for staining cell for cytometry analysis
6 cells/ml. 2. Transfer 100 µl of the solution (~1 x 10 5 cells) to a 5 ml culture tube. 3. Add 5-15 µg of purified recombinant Annexin V. The amount of purified recombinant Annexin V required to saturate binding sites may vary
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