Anti-Opsin, Red/Green; 100 μg

Anti-Opsin, Red/Green

The full range of color discrimination in humans is based on the presence and function of three cone photoreceptor mechanisms. Each cone type possesses a photo-sensitive pigment-protein complex consisting of 11-cis retinal and a unique opsin protein, which gives sensitivity in the short (S cone, peak sensitivity about 420nm), middle (M cone, peak sensitivity about 530nm with polymorphism; Winderckx et al., 1993; Neitz & Neitz, 1998), and long (L cone, peak sensitivity about 560nm with polymorphism; Neitz & Jacobs, 1990) wavelengths of the light spectrum. All three opsins are transmembrane proteins with seven membrane-spanning regions. Genes for the three types of cone opsins and the rod photoreceptor rhodopsin gene seem to be homologous with varying amounts of conservation. Strongest conservation is between the middle (green) and long (red) wavelength sensitive pigments on the X chromosome, suggesting a relatively recent duplication/divergence event (Nathans, 1989; Nathans et al., 1992). The S cone (blue) opsin is located on chromosome 7 and seems to have stronger conservation with rhodopsin. Cone photoreceptor distribution in humans is dominated by the M and L cone pigments.

Recognizes opsin, red/green.

Immunohistochemistry (Paraffin)

Immunohistochemistry:
1:200-1:300 on formalin-fixed, paraffin-embedded mouse retina tissue.

Optimal working dilutions must be determined by the end user.

• Human
• Mouse
• Monkey

Mouse. Reactivity with other species has not been determined

Recombinant human red/green opsin.

Red/Green

Retina

Purified rabbit polyclonal in buffer containing PBS, 0.02 M phosphate, 0.25 M NaCl, pH 7.6 with 0.1% sodium azide.

Stable for up to 6 months at 2-8°C in undiluted from date of receipt. Do not freeze. Keep vials well sealed to prevent evaporation.

• OPN1LW
• CBP
• CBBM
• RCP

Immunohistochemistry(paraffin) Analysis:
Opsin (cat. #: AB5405) staining pattern in mouse eye. Tissue pretreated with Citrate, pH 6.0. This lot of antibody was diluted to 1:200, using IHC-Select® Detection with HRP-DAB. Immunoreactivity is seen restricted to the cone shape cells responsible for photoreception.
Optimal Staining With Citrate Buffer, pH 6.0, Epitope Retrieval: Mouse Eye

Neuroscience

Sensory & PNS

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Chemicon (Millipore)

1 mg/mL

Polyclonal Antibody

Purified

Rabbit

Protein A chromatography