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- 2160
- 2025年07月09日
- Immunohistochemistry
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Immunohistochemistry
- 宿主：
0
- 抗原来源：
0
- 库存：
大量
- 是否单克隆：
0
- 规格：
50 mL
Autofluorescence Eliminator Reagent (Cat. No. 2160) Autofluorescence in monkey brain using a Cy3/TRITC filter before use of the Autofluorescence Eliminator Reagent.
Autofluorescence Eliminator Reagent (Cat. No. 2160). Elimination of autofluorescence in monkey brain using a Cy3/TRITC filter after the use of the Autofluorescence Eliminator Reagent.
Autofluorescence Eliminator Reagent (Cat. No. 2160) Autofluorescence in monkey brain using a Cy3/TRITC filter before use of the Autofluorescence Eliminator Reagent.
Autofluorescence Eliminator Reagent (Cat. No. 2160). Elimination of autofluorescence in monkey brain using a Cy3/TRITC filter after the use of the Autofluorescence Eliminator Reagent.
Autofluorescence Eliminator Reagent (Cat. No. 2160) Forty micron sections through the basal nucleus of Meynert from the brain of an adult human male. The sections were untreated (A), treated with the Autofluorescence Eliminator Reagent (B), or stained with a polyclonal goat anti-ChAT antibody (Cat. No. AB144P) and visualized with a Cy3-conjugated secondary antibody followed by treatment with the Autofluorescence Eliminator Reagent (C). Images were collected using a Bio-Rad Radiance 2100 MP Rainbow confocal microscope with a 20X objective. Illumination was provided by a 543 nm laser line, and emission was collected from 555-625 nm. The image collection settings (laser power, PMT gain, pinhole diameter, and background level) were identical for all three images. Photos courtesy of Michael Hendrickson and Ronald Kalil, W.M. Keck Laboratory for Biological Imaging, University of Wisconsin-Madison.
PROTOCOL:
1.After immunofluorescence histochemistry, the sections are immersed in PBS for 5 min.·
2.Sections are immersed in 70% ethanol for 5 min.·
3.Sections are immersed in Autofluorescence Eliminator Reagent for 5 min.
4.Sections are immersed in three changes of 70% ethanol for 1 min. each time.·
5.Sections are mounted using an antifading solution (e.g. DABCO, non-xylene-based).
Note: Reagent will block all fluorescence if incubation is too long; length of incubation time and number of 70% ethanol washes can be altered and optimized for various tissues and fluorescent antibody probes. In some instances the reagent can be applied prior to immunofluorescence, however in most cases better results are obtained when used as we direct above (use after immunofluorescence procedures are completed).
Optimal time of incubation with the Autofluorescence Eliminator Reagent should be determined by the end user for each sample.
Absorption maximum: 595-605
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