文献支持

Strep-Tactin® Superflow® high

capacity resin 纯化填料

价格询价

品牌IBA Lifesciences
产地德国
货号2-1208-010
更新日期2026年04月25日

IBA Lifesciences

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详细信息
询价记录
文献和实验
技术资料

保存条件：
2-8°C
保质期：
6 months
英文名：
Strep-Tactin® Superflow® high capacity 50% suspension
库存：
999+
供应商：
IBA Lifesciences
规格：
20 ml

Strep-Tactin^® Superflow^® high capacity is a resin for purification of strep-tagged proteins <90 kDa via affinity chromatography. The product is optimized for column-based use and suitable for HPLC/FPLC. It consists out of highly cross-linked agarose beads that are coupled to Strep-Tactin^®, which is a streptavidin variant. Strep-Tactin^® binds to proteins that were fused to a Strep-tag^®II or a Twin-Strep-tag^®, enabling a straightforward purification of tagged proteins from mixed samples, reaching a purity of >95%. Due to the targeted elution, tedious titration steps are not necessary. In addition, regeneration allows for multiple use of the resin.
In comparison to Strep-Tactin^® Superflow, the high capacity version has a higher density of Strep-Tactin^®, making it suitable for highly expressed proteins. This resin is also an alternative for proteins that are difficult to elute from our Strep-Tactin^®XT 4Flow^® resins.

Please note that biotinylated proteins also bind to Strep-Tactin^® Superflow^® high capacity. If biotinylated proteins are expected, use BioLock. Furthermore, this resin is a 50% suspension, so for packing e.g. a 1 ml gravity column, 2 ml of resin is required.

For further information about our different resins including recommendations, check our resin specifications.

Specifications

Bead Size:	60 - 160 µm spherical
Biotin Binding Capacity:	> 900 nmol/ml resin
Dynamic Protein Binding Capacity:	7 mg/ml resin
Exclusion Limit:	6 x 10^6
Form:	50% suspension
Possible Application:	Protein purification of Strep-tag^®II and Twin-Strep-tag^® fusion proteins
Specificity:	Twin-Strep-tag^®; Strep-tag^®II; cross reactivity: biotin
Support:	6% agarose, crosslinked

Shipping information

Storage:	2-8 °C
Stability:	6 months after shipping
Shipping:	Room temperature

货号	规格
2-1208-002	4 ml
2-1208-010	20 ml
2-1208-025	50 ml
2-1208-500	1000 ml

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作者 内容 询问日期

文献和实验

该产品被引用文献

[1] Ng T L, Olson E J, Yoo T Y, et al. High-content screening and computational prediction reveal viral genes that suppress the innate immune response[J]. Msystems, 2022, 7(2): e01466-21.

[2] Fang W, Bartel D P. MicroRNA clustering assists processing of suboptimal microRNA hairpins through the action of the ERH protein[J]. Molecular cell, 2020, 78(2): 289-302. e6.

[3] Ramirez N G P, Lee J, Zheng Y, et al. ADAP1 promotes latent HIV-1 reactivation by selectively tuning KRAS–ERK–AP-1 T cell signaling-transcriptional axis[J]. Nature communications, 2022, 13(1): 1109.

[4] Ryu J K, Katan A J, van der Sluis E O, et al. The condensin holocomplex cycles dynamically between open and collapsed states[J]. Nature structural & molecular biology, 2020, 27(12): 1134-1141.

相关实验

样品体积增加后，对蛋白质的纯化效率有怎样的影响？
，而总体的回收效率却比较低。 02 对比洗脱得到蛋白量如果转而将洗脱得到蛋白量加以对比，可以发现标准 Ni-NTA 柱结合的蛋白量最多，当然这得益于其填料特性——优异的结合载量。(CAPACITY – STANDARD Ni-NTA INFLUENCED BY LOADING VOLUME) 但如果上样体积 120CV，其与 Strep-Tactin® XT 的差异并不明显。同时，Karthaus 和他的团队也提出了这样的问题：样品中如果蛋白含量不同，是否还会得出以上结论呢？ 03 样品中
对比 Strep-tag 与 His-tag 系统异同点？
技术则成为首选，因其µM-pM的亲和力范围赋予了较大优势。因此根据需要选择合适的纯化系统非常重要。此外，由于不同的实验需求，需要多种多样的产品形式。Strep-tag®系统优点众多，历经长时间的发展，市面上已不仅有纯化填料可以选择，分析类实验的工具也日渐完善。在各类文献中不难看到的有：标记了荧光染料的Strep-Tactin®XT偶联物/抗体，Strep-Tactin®XT包被板，和适用于SPR分析的Twin-Strep-tag® Capture Kit。
聊一聊蛋白纯化都在讨论的质与量
的非特异性蛋白。当进行类似分析等有高亲和力/高特异性抗体需求的实验应用时，如 SPR 或 BLI，Strep-tag® 技术则成为首选，因其 µM-pM 的亲和力范围赋予了较大优势。因此根据需要选择合适的纯化系统非常重要。此外，由于不同的实验需求，需要多种多样的产品形式。Strep-tag® 系统优点众多，历经长时间的发展，市面上已不仅有纯化填料可以选择，分析类实验的工具也日渐完善。在各类文献中不难看到的有：标记了荧光染料的 Strep-Tactin® XT 偶联物/抗体，Strep