- 询价
- IBA Lifesciences
- 德国
- 2-1209-550
- 2026年04月15日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
2-8°C
- 保质期：
6 months
- 英文名：
Strep-Tactin® Superflow® high capacity column
- 库存：
999+
- 供应商：
IBA Lifesciences
- 规格：
5* 0.2ml
Strep-Tactin® Superflow® high capacity columns are ready-to-use for purifying strep-tagged proteins below 90 kDa via affinity chromatography. These gravity flow columns contain a highly cross-linked agarose coated with streptavidin variant Strep-Tactin®, which captures proteins fused to a Strep-tag®II or a Twin-Strep-tag®. A straightforward purification principle results into a highly pure (>95%) protein preparation. After regeneration, the columns can be used multiple times.
Please note that biotinylated proteins also bind to Strep-Tactin® Superflow® high capacity. If biotinylated proteins are expected, use BioLock. Furthermore, if large (sample and/or buffer) volumes have to be applied, we recommend our WET FRED system.
For a resin overview with recommendations, browse our resin specifications.
Specifications
| Bead Size: | 60 - 160 µm spherical |
|---|---|
| Biotin Binding Capacity: | > 900 nmol/ml resin |
| Dynamic Protein Binding Capacity: | 7 mg/ml resin |
| Exclusion Limit: | 6 x 10^6 |
| Form: | Pre-packed gravity flow column |
| Possible Application: | Protein purification of Strep-tag®II and Twin-Strep-tag® fusion proteins |
| Specificity: | Twin-Strep-tag®; Strep-tag®II; cross reactivity: biotin |
| Support: | 6% agarose, crosslinked |
Shipping information
| Storage: | 2-8 °C |
|---|---|
| Stability: | 6 months after shipping |
| Shipping: | Room temperature |
| 货号 | 规格 |
| 2-1209-550 | 5* 0.2ml |
| 2-1209-001 | 1 ml |
| 2-1209-051 | 5 ml |
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- 内容
- 询问日期
文献和实验[1] Chang H H, Huang L C, Browning K S, et al. The phosphorylation of carboxyl-terminal eIF2α by SPA kinases contributes to enhanced translation efficiency during photomorphogenesis[J]. Nature Communications, 2024, 15(1): 3467.
[2] Paik I, Chen F, Ngoc Pham V, et al. A phyB-PIF1-SPA1 kinase regulatory complex promotes photomorphogenesis in Arabidopsis[J]. Nature Communications, 2019, 10(1): 4216.
[3] Gao Y Q, Jimenez-Sandoval P, Tiwari S, et al. Ricca’s factors as mobile proteinaceous effectors of electrical signaling[J]. Cell, 2023, 186(7): 1337-1351. e20.
Expi Supernatant 进行培养。 纯化过程中，选择柱体积为 0.2 ml 的三种纯化柱：Ni Sepharose 6 FastFlow, Ni Sepharose Excel, 和 Strep-Tactin®XT 4Flow®。 分别用其纯化 60CV (column volume, 柱体积) 和 120CV 的样品，并保持上样样品中的蛋白质含量在纯化柱最大结合载量 80%-95 %。 二、实验结果 01 对比蛋白质回收效率 对于不同的填料，可以从洗脱组分中回
步骤进行比较 (以 Twin-Strep-tag®-mCherry 为例，左方纯化柱为 Gravity flow Strep-Tactin® Superflow® column，#2-1207-001；右方为 Gravity flow Strep-Tactin®XT Superflow® column，#2-4012-001)。 检验 Strep-Tactin®/Strep-Tactin®XT 的纯化结果 纯化完成后，使用 SDS-PAGE 分析所收集的不同样本，图
。图 A图 A：分别向 Strep-Tactin®XT High Capacity 1ml 重力柱（左）和 Ni-NTA 1ml 重力柱（右）中，同时加入含 4mg mCherry-Twin-Strep-tag (TST) 或 mCherry-His-tag (His) 萤光蛋白的 ExpiCHO 细胞培养上清（注: 接下来的实验皆是取哺乳细胞表达某抗体后的上清，另加入高纯度目标蛋白进行实验)。由图可见，Strep-Tactin®XT（左）能高效率的捕捉 mCherry-TST，而右方
