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- STEMCELL Technologies
- 05876
- 2026年01月06日
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产品概要
mTeSR™1 is the most widely published feeder-free cell culture medium for human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPS cells), with established protocols for applications ranging from derivation to differentiation. It has been used to successfully maintain hundreds of ES and iPS cell lines in over 40 countries, and has supported top pluripotent stem cell publications and researchers. mTeSR™1 is a highly specialized, serum-free, and complete cell culture medium. With pre-screened raw materials that ensure batch-to-batch consistency and robust feeder-free protocols for ES and iPS cell culture, mTeSR™1 provides more consistent cultures with homogeneous, undifferentiated phenotypes.mTeSR™1 is now available without phenol red, for applications in which the presence of phenol red is undesirable.
Components:
- mTeSR™1 Without Phenol Red Basal Medium, 400 mL
- mTeSR™1 5X Supplement, 100 mL
多能干细胞
Species:
人
Application:
细胞培养; 扩增; 维持培养
Formulation:
无血清
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文献和实验Feeder-Independent Culture Systems for Human Pluripotent Stem Cells
and feeder-independent culture media has provided a platform for greater reproducibility and standardization in this field. Here we provide detailed protocols for the use of mTeSR™ 1 and TeSR™2 with various cell culture matrices as well as defined
/iPS细胞的长期培养提供了所需的优化ECM包被，并支持在多种无血清/无饲养层人ES/iPS扩增培养基（例如，mTeSR®、PluriSTEM™）中培养人ES/iPS细胞。以下是使用干细胞合格的ECM凝胶基质包被6孔板的一般指南。 所有操作程序均应在生物安全柜中的无菌条件下进行。 1.使用前一天，在2-8 °C冰箱中解冻ECM凝胶基质（CC131）。解冻后，将ECM凝胶始终置于冰上，并使用预先冷却的培养基和移液器，以避免产品凝胶化。 2.用冷的DMEM/F12（D6421）培养基稀释ECM凝胶
Lentiviral Transduction Protocol for Human Embryonic Stem Cells
设备 1. 24-well culture dishes (Product No. CLS3526) 2. Matrigel™ basement membrane 实验材料 MISSION® shRNA lentiviral particles 实验步骤 Prior to transduction — establish the Puromycin Kill Curve
技术资料