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文献和实验SQ Blood Mini Protocol for 50ul Clotted Blood
2. Nuclease-free 1.5 ml microcentrifuge tubes 3. Water Bath preset at 37°C 实验步骤 1. Transfer the 50ul blood include any liquid residual into a 1.5 ml centrifuge tube. 2. Add 550ul WTL Buffer and pipet
is cDNA concentration 200 ng/ul too high for qRT-PCR-Rea
The gene we are interested in looking at is not very abundant. At 100 ng/ul, the CT value is 30.9 In the literature that I have looked at, people seems to using much lower levels of cDNA. I would actually
Mag-Bind Blood RNA Isolation Protocol (for 50ul Blood)
1. Prepare a Lysis Mixture by mixing one volume of Buffer MRB and one volume of isopropanol. For 96 samples, combine15 ml Buffer MRB with 15 ml isopropanol and mix throughly by vortexing. Add 300 ul Lysis Mixture for each sample at step 3. 2. Add 50 ul
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