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Anti-Phospho-Histone H3 (Ser10

), clone 3H10, FITC Conjugate; 100 μg
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  • 询价
  • 16-222
  • 2025年07月15日
  • Immunofluorescence;Western Blotting
  • Mouse
  • Human
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      Immunofluorescence;Western Blotting

    • 宿主：

      Mouse

    • 库存：

      大量

    • 抗原来源：

      0

    • 适应物种：

      Human

    • 是否单克隆：

      1

    • 规格：

      100 μg

    Product Images

    IF: Mitotic HeLa cells stained with 4μg/ml of this lot (green) and DAPI (blue).

    WB: Acid extract from untreated (Lane 1) and colcemid treated (Lane 2) HeLa cells were probed with anti-phospho-Histone H3, clone 3H10, FITC Conjugate...
    Species Reactivity Key Applications Host Format Antibody Type
    H WB, IC, IF Mouse Purified Monoclonal Antibody
    Description:
    Anti-Phospho-Histone H3 (Ser10), clone 3H10, FITC Conjugate
    Background Information:
    Histone H3 is one of the five main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N-terminal tail, H3 is involved with the structure of the nucleosomes of the 'beads on a string' structure. The N-terminal tail of histone H3 protrudes from the globular nucleosome core and can undergo several different types of epigenetic modifications that influence cellular processes. These modifications include the covalent attachment of methyl or acetyl groups to lysine and arginine amino acids and the phosphorylation of serine or threonine.
    Alternate Names:
    • H3 histone family, member T
    • histone 3, H3
    • histone cluster 3, H3
    Clone:
    clone 3H10
    Specificity:
    Recognizes histone H3 phosphorylated at Ser10, MW 17 kDa.
    Key Applications:
    • Western Blotting
    • Immunocytochemistry
    • Immunofluorescence
    Application Notes:
    Western Blot Analysis: 0.5-5 µg/mL of this lot detected phosphorylated histone H3 in acid extracted proteins from mitotic HeLa cells (Catalog # 17-306) treated with colcemid.
    Species Reactivity:
    Human
    Species Reactivity Note:
    Human. Broad species cross-reactivity is expected.
    Isotype:
    IgG
    Immunogen:
    A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10.
    Epitope:
    Ser10
    Modifications:
    Phosphorylation
    Molecular Weight:
    17 kDa
    Control:
    Mitotic HeLa cells (IF).
    Presentation:
    Purified mouse monoclonal IgG in buffer containing PBS with 0.05% sodium azide, pH7.1.
    Storage Conditions:
    Stable for 1 year at from date of receipt.
    Gene Symbol:
    • HIST3H3
    • H3FT
    • H3/t
    • H3t
    • MGC126888
    • H3/g
    • H3.4
    • H3T
    • MGC126886
    Quality Assurance:
    Immunocytochemistry: Mitotic HeLa cells showed positive chromosome staining with 4μg/mL of this antibody.
    Antibody Category:
    Epigenetics & Nuclear Function
    Antibody Sub-Category:
    Histones
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Upstate (Millipore)
    Antibody Type:
    Monoclonal Antibody
    Format:
    Purified
    Host:
    Mouse

    Product Family Information

    Histone H3

    Millipore’s Anti-Histone H3 Antibody demonstrates specificity against Histone H3. See below for data, references and related products for Histone H3. All Millipore antibodies are based on the expertise of Upstate & Chemicon.

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    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    A proprietary immunogen based on a peptide sequence containing phospho-serine corresponding to residue 10 of human histone H3. Clone 3H10. 详细描述见链接：http://www.millipore.com/catalogue/item/16-222

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    图标文献和实验
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      solution contains 100 mMMgCl 2 , 1 mM un-labelled ATP, and approximately 30 MBq/mL [g- 32 P]ATP. 5. Phospho-glycogen synthase 2 (pGS2) peptide is available fromUpstate (#12-241) and the pGS2 10× stock solution contains200 μM pGS2 in kinase buffer

    • Protocol for intracytoplasmic staining of cytokines for FACS analysis

      ; Pharmingen 18195A) for FL2 anti-IL10 (JES5.16E3-PE; Pharmingen 18435A) for FL1 anti-TGF-beta (rabbit anti pan TGF-beta; R&D Systems AB-100-NA home made FITC conjugate) for FL2 8) Wash extensively in saponin buffer followed by PBS + 0.1% azide

    • Cell Surface Immunofluorescence Staining Protocol

      . In the mouse, purified anti-mouse CD16/CD32 antibody specific for Fcγ R III/II (BioLegend Cat. #101302, clone 93) can be used to block nonspecific staining of antibodies. In this case, block Fc receptors by pre-incubating cells with 5-10 μg/ml purified anti-CD

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