Anti-Myc Tag, clone 4A6, HRP c

Anti-Myc Tag, clone 4A6, HRP conjugate

Myc-Tag is a short synthetic peptide sequence derived from C-terminal region of human c-myc that is used as an epitope tag. The Myc tag can be cloned either upstream or downstream of the target gene, resulting in a fusion protein that allows efficient purification, detection, and localization of target proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. The myc epitope tag is widely used to detect expression of recombinant proteins in bacterial, yeast, insect, and mammalian systems.

• Transcription factor p64
• avian myelocytomatosis viral oncogene homolog
• myc proto-oncogene protein
• v-myc avian myelocytomatosis viral oncogene homolog
• v-myc myelocytomatosis viral oncogene homolog (avian)

4A6

Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

Western Blotting

Human

Human. Other species cross-reactivity not tested.

KLH-conjugated, synthetic peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc. Clone 4A6.

C-terminal

Varies depending upon the protein which is tagged.

Positive control for human Myc: Widespread expression including A431 cell lysate, ovarian cancer cell lysate, or breast carcinoma tissue. Myc fusion protein expressed in cells.

Purified mouse monoclonal IgG1 conjugated to horseradish peroxidase (HRP) in buffer containing: 50% storage buffer (PBS, 0.05% Kathon®, 0.2 mg/mL BSA) and 50% glycerol.

Stable for 1 year at -20ºC from date of receipt.

• MYC
• C-MYC
• c-Myc

Evaluated by Western Blot on Myc-PP2A A subunit transfected NIH/3T3 lysates.

Western Blot Analysis:
1:500 dilution of this antibody detected myc-PP2A on 10 µg of Myc-PP2A A subunit transfected NIH/3T3 lysates.

Epitope Tags & General Use

Epitope Tags

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Upstate (Millipore)

Variable

Monoclonal Antibody

Purified

Mouse

Immunogen: peptide corresponding to amino acids 410-420 (MEQKLISEEDL) of human Myc

Specificity: Recognizes and is specific for recombinant proteins containing the Myc epitope tag (EQKLISEEDL) in a variety of sequence contexts. Also recognizes human Myc.

Species Cross-reactivity: Human, others not tested

Ig Type: IgG

Host: Mouse

Molecular Weight: Varies depending upon the protein which is tagged