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NovaSeal™ Crimping SolutionThe NovaSeal crimping solution is unique; the tool crimps and cuts a pinch-pipe and tube at the same time in one simple motion. |
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/NM0040
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文献和实验Extraction and PCR Analyis of DNA from Mice Tails
or heat for 1hour at 65℃ to dissolve. Optional: measure OD260 and use about 8µg DNA per lane if performing Southern analysis. For PCR analysis,run thermocycler at (95℃x1min,63℃x1min,72℃x1min)x30; 72℃x5min; 4℃ end for,say,19-22-mer primer pairs
LABELING ALPHA-ACTININ WITH IATR
of injection buffer)and reading OD555. D/P = {OD555 x 41 / 60,000} / {(mg/ml)/ 100,000} 13.Dilute to 5 mg/ml for microinjection if necessary.Extra conjugated alpha-actinin can be stored as aliquots in liquid N2.
the following DNA dilution, and aliquot 35 μl into ten 1.5 ml microcentrifuge tubes: DNA 100 μg 10X TM buffer 35 μlsterile ddH2 O q.s. Final Volume 350 μl 2. To determine the optimal sonication conditions, sonicate the DNA samples in five of the tubes
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