产品封面图

Anti-Tyrosine Hydroxylase; 100

μL
收藏
  • 询价
  • 2025年12月26日
  • ELISA;Immunoprecipitation
  • Rabbit
  • Drosophila;Ferret;Human
avatar
默克生命科学
11 年金牌会员

  • 企业认证

    点击 QQ 联系

    • 相关产品推荐更多 >

    万千商家帮你免费找货

    0 人在求购买到急需产品
    • 详细信息
    • 文献和实验
    • 技术资料
    • 应用范围：

      ELISA;Immunoprecipitation

    • 宿主：

      Rabbit

    • 适应物种：

      Drosophila;Ferret;Human

    • 库存：

      大量

    • 抗原来源：

      0

    • 是否单克隆：

      2

    • 规格：

      100 μL

    Close

    Rabbit anti-Tyrosine Hydroxylase (Catalog Number AB152) localization of tyrosine hydroxylase in culture of primary neurons. Photo courtesy of Dr. Mehdi Doroudchi, Avigen.

    anti-Tyrosine Hydroxylase (TH) staining in mouse primary neural cultures using AB152 shown with an FITC fluorescent secondary (green). Fixation was 4% PFA and the primary antibody incubation was 1:1000, overnight at 4°C.

    Anti-Tyrosine Hydroxylase (TH) labelled mouse primary neural cultures using AB152 and a FITC labelled secondary (green). Fixation was 4% PFA and the primary antibody incubation was 1:1000, overnight at 4°C.

    Rabbit anti-Tyrosine Hydroxylase (Catalog Number AB152) staining of rat brain stem.

    TH (PN: AB152) staining on normal substantia nigra. Tissue pretreated with Citrate, pH 6.0 antigen retrieval. Polyclonal Ab. diluted to 1:300, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern to include cell body and synaptic nerve terminals of dopaminergic & noradrenergic neurons. Low Mag.

    TH (PN: AB152) staining on normal substantia nigra. Tissue pretreated with Citrate, pH 6.0 antigen retrieval. Polyclonal Ab. diluted to 1:300, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern to include cell body and synaptic nerve terminals of dopaminergic & noradrenergic neurons. High Mag.

    Western Blot Analysis:
    PC12 lysate was resolved by electrophoresis, transferred to PVDF membrane and probed with anti-tyrosine hydroxylase (1:1000 dilution of this lot).
    Proteins were visualized using a donkey anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.

    Arrow indicates protein tyrosine hydroxylase (~62 kDa).

    Species Reactivity Key Applications Host Format Antibody Type
    H, R, M, Fe, Ft, Sqd, Dr, Ml ELISA, IF, IH, IH(P), IP, WB Rabbit Affinity Purified Polyclonal Antibody
    Description:
    Anti-Tyrosine Hydroxylase
    Background Information:
    Tyrosine hydroxylase plays an important role in the physiology of adrenergic neurons. It is the first and rate-limiting enzyme involved in the biosynthesis of the catecholamines Dopamine and Norepinephrine from tyrosine. TH is, therefore, a useful marker for dopaminergic and noradrenergic neurons. The enzymatic activity of TH requires ferrous ions as cofactors and is believed to be regulated by phosphorylation. At least four isoforms of human TH have been identified which result from alternative splicing.
    Alternate Names:
    • Tyrosine 3-hydroxylase
    • Tyrosine 3-hydroxylase
    • tyrosine hydroxylase
    Specificity:
    Tyrosine hydroxylase. By western blot, AB152 selective labels a single band at approximately 62kDa (reduced) corresponding to Tyrosine Hydroxylase. It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
    Key Applications:
    • ELISA
    • Immunofluorescence
    • Immunohistochemistry
    • Immunohistochemistry (Paraffin)
    • Immunoprecipitation
    • Western Blotting
    Application Notes:
    Immunohistochemistry:
    A previous lot of this antibody was uesd at 1:100-1:1,000 dilution, 4°C, for 1-3 days. Stains both fresh frozen and paraffin embedded tissue samples by indirect immunofluorescence and immunoperoxidase.

    Immunofluorescence:
    A previous lot of this antibody was used at 1:100-1:1,000 dilution.

    Immunoprecipitation:
    A previous lot of this antibody was used on Immunoprecipitation.

    ELISA:
    A previous lot of this antibody was used in ELISA.

    Optimal dilutions must be determined by the end user.
    Species Reactivity:
    • Human
    • Rat
    • Mouse
    • Feline
    • Ferret
    • Squid
    • Drosophila
    • Mollusk
    Species Reactivity Note:
    Rat, mouse, ferret, feline, Aplysia (marine mollusc). It is expected that the antibody will react with most mammalian and many non-mammalian species. It has been reported that this antibody does not work on paraffin embedded human tissue.
    Immunogen:
    Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate).
    Molecular Weight:
    62 kDa
    Control:
    POSITIVE CONTROL: Brain (corpus striatum, sympathetic nerve terminals) and adrenal glands.
    NEGATATIVE CONTROL: Liver.
    Presentation:
    Purified rabbit polyclonal in buffer containing liquid in 10 mM HEPES (pH 7.5), 150 mM NaCl (pH 7.5), 100 ug/mL BSA and 50% glycerol.
    Storage Conditions:
    Maintain frozen at -20ºC to -70ºC in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. Do not store in a self defrosting freezer.
    Gene Symbol:
    • TH
    • TYH
    • EC 1.14.16.2
    Quality Assurance:
    Routinely evaluated by Western Blot on PC12 lysates.

    Western Blot Analysis:
    1:1000 dilution of this lot detected tyrosine hydroxylase on 10 μg of PC12 lysates.
    Antibody Category:
    Neuroscience
    Antibody Sub-Category:
    • Neurotransmitters & Receptors
    • Neuronal & Glial Markers
    Usage Statement:
    Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    Trade Name:
    Chemicon (Millipore)
    Format:
    Affinity Purified
    Host:
    Rabbit
    Antibody Type:
    Polyclonal Antibody

    更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com

    Denatured tyrosine hydroxylase from rat pheochromocytoma (denatured by sodium dodecyl sulfate). 详细描述见链接：http://www.millipore.com/catalogue/item/AB152

    风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。

    图标文献和实验
    相关实验

    • Phenylalanine Hydroxylase, Tyrosine Hydroxylase, and Tryptophan Hydroxylase

      There are four enzymes that utilize reduced pteridine as an electron donor and incorporate one atom of oxygen into their substrates. They are phenylalanine 4-monooxygenase (phenylalanine hydroxylase, PAH, EC 1.14.16.l), tyrosine 3-monooxygenase

    • Quantification of Tyrosine Hydroxylase mRNA

      The main biochemical characteristic of Parkinson’s disease (PD) is reduction of the neurotransmitter dopamine and the dopamine-synthesizing enzyme system, including tyrosine hydroxylase (TH, tyrosine 3-monooxygenase, EC 1.14.16.2

    • 人S蛋白100B(S-100B)酶联免疫分析

      孔，在第一、第二孔中分别加标准品 100μl ，然后在第一、第二孔中加标准品稀释液 50μl ，混匀；然后从第一孔、第二孔中各取 100μl 分别加到第三孔和第四孔，再在第三、第四孔分别加标准品稀释液 50μl ，混匀；然后在第三孔和第四孔中先各取 50μl 弃掉，再各取 50μl 分别加到第五、第六孔中，再在第五、第六孔中分别加标准品稀释液 50ul ，混匀；混匀后从第五、第六孔中各取 50μl 分别加到第七、第八孔中，再在第七、第八孔中分别加标准品稀释液 50μl ，混匀后从第七、第八孔中

    查看更多相关实验 >
    图标技术资料

    暂无技术资料 索取技术资料

    同类产品报价

    产品名称
    产品价格
    公司名称
    报价日期
    Anti-Tyrosine Hydroxylase; 100 μL
    询价
    默克生命科学
    2024年04月07日询价
    Anti-Tyrosine Hydroxylase antibody [TH-100]
    ￥3996
    上海瑶韵生物科技有限公司
    2025年07月12日询价
    Anti-Tyrosine Hydroxylase
    ￥1380
    上海泽叶生物科技有限公司
    2025年06月19日询价
    Anti-Tyrosine Hydroxylase
    ￥3149
    苏州瑞诺德生物科技有限公司
    2025年01月19日询价
    Rabbit anti-Tyrosine Hydroxylase Polyclonal Antibody,Tyrosine Hydroxylase 抗体
    ￥1500
    爱必信(上海)生物科技有限公司
    2025年06月29日询价
    Anti-Tyrosine Hydroxylase; 100 μL
    询价