(±)4-Hydroxymephenytoin

麻醉家兔、大鼠左室内压（LVP）与左室内压变化速率（±dp/dtmax）的测定

心脏左心室内压（left ventricular pressure LVP）及其变化速率，是反映和评价左心室收缩功能与舒张功能的重要指标，不论是在临床的心导管检测，还是在基础医学的实验教学与科研中经常要用到这一测量方法。一般情况下，家兔、大鼠均采用通过颈总动脉插管到达心脏左心室的方法，即可获得反映左心室收缩功能与舒张功能的指标，而且操作简便、易行。另外大鼠还可以采用经左心室心尖部插管到达左心室，测量反映左心室收缩功能与舒张功能指标的方法。这一测量方法，可以在测量左心

CYP2C19-Mediated (S)-Mephenytoin 4-Hydroxylation Assayed by High-Performance Liquid Chromatography With Radiometric Detection

A reverse-phase, high-performance liquid chromatography method is described for the quantification of 4′-hydroxymephenytoin formed enzymatically from 14 C-labeled (S )-mephenytoin following incubation with cDNA-expressed CYP2C19 or human

CYP2Cl g-Mediated (S)-Mephenytoin 4-Hydroxylation Assayed by High-Performance Liquid Chromatography with Radiometric Detection

indicator of the catalytic activity of cDNA-expressed CYP2C 19 (2 ). Varrous analytical methods have been developed to measure the enzymatic formation of 4’-hydroxymephenytoin, including isocratrc, reversed-phase hrgh performance hqutd chromatography