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文献和实验rongshenghao 加入激活剂或抑制剂后打算用Fura-2或者Fluo-3测量细胞内钙离子浓度 问： 1.Fura-2和Fluo-3区别？ 2.测量的是游离钙还是总钙呀？ 3.还有什么需要注意的？ 谢了！ 2010tiger Fura-2采用双波长检测，Fluo-3是单波长检测，Fura-2的激发波长是340nm和380nm，发射波长为510nm。Fluo-3的激发波长是506nm
Measurement of [Ca2+]i in Whole Cell Suspensions Using Fura-2
Use of Fura-2 in whole cell suspensions to measure changes in intracellular Ca2+ is probably one of the simplest, yet most widely used protocols described in this volume. Whole cell suspensions are loaded with Fura-2 and then placed
Measurement of Intracellular Free Calcium Ion Concentration in Cell Populations Using Fura- 2
, which cleaved the ester bond and left the calcium-sensitive free acid trapped within the cell (5 ). A number of improved calcium indicators have since been developed (e.g., fura-2, indo-1, and fluo-3), but the basic principles of dye loading and continuous
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