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- 2025年07月16日
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文献和实验8 eggs per day, day 7- day 13 cut CAM, wash in precooled PBS, in 10 ml WASH 1 (PBS, 5 mM EDTA, COMPLETE) on ice cut in pieces in petri dish on ice centrifuge 4 min at 1000 rpm (table top centifuge, Falcon 15 ml tubes) at 4°C
Chorioallantoic Membrane (CAM) Assay
8 eggs per day, day 7- day 13 cut CAM, wash in precooled PBS, in 10 ml WASH 1 (PBS, 5 mM EDTA, COMPLETE) on ice cut in pieces in petri dish on ice centrifuge 4 min at 1000 rpm (table top centifuge, Falcon 15 ml tubes) at 4°C
Immunohistochemistry of p53, Bcl-2, and Ki-67 as Predictors of Chemosensitivity
: LSAB method) was used to assess expression of p53 protein, Bcl-2 protein, and Ki-67. A chemosensitivity test was carried out with the histoculture drug response assay method using four drugs: mitomycin C, 5-fluorouracil, doxorubicin hydrochloride (ADM
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