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文献和实验Construction of Capsid-Modified Recombinant Bovine Adenovirus Type 3
adenoviruses an attractive alternative. Moreover, readministration of viral vectors is often required to maintain therapeutic levels of transgene expression, resulting in vector-specific immune responses. Although a number of features of bovine adenovirus (BAdV
The Use of Electroporated Bovine Spermatozoa to Transfer Foreign DNA into Oocytes
(1 ,2 ), as well as to look at the consequences of oncogene expression (3 ,4 ). Through this technique, recombinant DNA molecules of any type can be introduced into zygotes for use in producing transgenic domestic animals. Introduction of foreign genes into early embryos
DETECTION OF RECOMBINANT PROTEINS BY ELISA
of interest. If the recombinant protein is not soluble in H2 O, dissolve the insoluble pellet in a volume of 7 M urea equal to 1/10 of the bacterial culture volume. Vortex the samples at a medium speed for about 30 minutes (use a vortex setting of 4.5 on the Fisher Vortex Genie
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