Prostaglandin E1

Purification of E1 and E1-Like Enzymes

physical and mechanistic features of the E1 family appear well conserved. Formation of an obligatory E1-ubiquitin thiol ester intermediate forms the basis of a one-step covalent purification of the enzyme on ubiquitin-linked affinity columns

人CYP2E1酶联免疫分析

人CYP2E1 酶联免疫分析 试剂盒使用说明书 本试剂仅供研究使用         目的：本试剂盒用于测定人血清，血浆及相关液体样本中 CYP2E1 的含量。 实验原理：   本试剂盒应用双抗体夹心法测定标本中人 CYP2E1 水平。用纯化的人 CYP2E1 抗体包被微孔板，制成固相抗体，往包被单抗的微孔中依次加入 CYP2E1 ，再与 HRP 标记的 CYP2E1 抗体结合，形成抗体 - 抗原 - 酶标抗体复合物，经过彻底洗涤

Immunoprecipitation of E1 A-Containing Protein Complexes

of monoclonal antibodies and the fortuitous properties of nonionic detergents. These highly efficient and specific means of isolating protein complexes from cell lysates, while detailed here for E1Acontaining protein complexes, have general application