• 我要登录|
  • 免费注册
    |
  • 我的丁香通
    • 企业机构:
    • 成为企业机构
    • 个人用户:
    • 个人中心
  • 移动端
    移动端
丁香通 logo丁香实验_LOGO
搜实验

    大家都在搜

      大家都在搜

        0 人通过求购买到了急需的产品
        免费发布求购
        发布求购
        点赞
        收藏
        wx-share
        分享

        Immunoprecipitation of E1 A-Containing Protein Complexes

        互联网

        506
        Important insights into cell growth and transcriptional regulation have been realized by correlating adenovirus ElA-induced cell-growth-control mechanisms with ElA’s specific interactions with host-cell proteins (see ref. 4 ). A key method for directly demonstrating these ElA-host cell protein interactions is by immunoprecipitation. This assay is performed under conditions that efficiently lyse cells, but preserve protein-protein interactions. This method combines the elegant specificities of monoclonal antibodies and the fortuitous properties of nonionic detergents. These highly efficient and specific means of isolating protein complexes from cell lysates, while detailed here for E1Acontaining protein complexes, have general application for the study of other cellular-protein complexes.
        ad image
        提问
        扫一扫
        丁香实验小程序二维码
        实验小助手
        丁香实验公众号二维码
        扫码领资料
        反馈
        TOP
        打开小程序