2,4-Pyridinedicarboxylic Acid

Biotinylation of a Propargylated Cyclic (3′‐5′) Diguanylic Acid and of Its Mono‐6‐Thioated Analog Under “Click” Conditions

?cyanoethylthio group is effected by treatment with 2,4,6?triisopropybenzenesulfonyl chloride and triethylamine to give a 6?(2,4,6?triisopropylbenzenesulfonic acid) ester intermediate. Reaction of this key intermediate with 3?mercaptoproprionitrile

Protocol for Silver Staining

and you are not able to scale up your isolation of protein to reach coomasie stainable levels,you must use a mass spec compatible silver stain protocol.You must only use methanol and acetic acid during the fixing step.You must not use any solutions containing

酶法、热修复、微波法修复抗原

.0 ml Ultra-pure water 978.0 ml 0.5% sodium hydroxide solution 0.5% hydrochloric acid solution Xylene Industrial methylated spirits (IMS) or methanol Method Dewax and re-hydrate the paraffin sections by placing them in 3 changes