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文献和实验A Method to Assay Inhibitors of DNA Polymerase IIIC Activity
, one of these, designated pol III, is responsible for DNA replication and growth of bacteria and, therefore, warrants consideration as a drug target. However, the pol III enzymes of Gram-positive and Gram-negative species are quite different, and the Gram-positive enzyme
DNA Polymerase Inhibition Assay (PIA) for the Detection of DrugDNA Interactions
The authors have devised a simple method for the detection of drug–DNA interaction and sequence selectivity in vitro (1 ).The method is based on the inhibition or termination of bacterial DNA polymerase(s) when it encounters modified DNA
Cloning Polymerase Chain Reaction Products Utilizing theT/A Overhang and a Kit
to the ligation reaction mixture. This procedure is based on the fact that Taq polymerase has a nontemplate dependent activity that adds a single deoxyadenosine to the 3′ ends of double-stranded DNA (2 ). The Invitrogen pCR™II vector is supplied as a linearized
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