Arachidonic Acid ethyl ester

Neutralizing Arachidonic Acid

NOTE - Use arachidonic acid from Biomol (# FA-003) 1) Make up the appropriate concentration of AA in 100% ethanol. 2) Add 1µl of phenol red solution. --> The solution should appear yellow since the free acid

Arachidonic Acid Labeling

with ITS (4 ml/L should yield 5 mg/L insulin and 5 mg/L transferrin). --> "Appropriate" = bring cells to a final concentration of 5 X 105 cells/ml. 4) Add [3H] arachidonic acid to get ~2 µCi/ml (2 X 106 µCi/ml???). --> Make sure final AA

磷酸酯 phosphoric acid ester，phosphoric ester

磷酸结合在醇羟基上的化合物。磷酸因为是三碱基酸，可能有单酯、二酯、三酯三种，在生物体内存在着前二种。作为生物成分，或代谢中间产物可广泛见到。核酸、磷脂质是二酯形式。糖的酯是糖代谢的中间产物，核苷酸是核苷的磷酸酯。