- 询价
- 06-418
- 2025年07月10日
- Chromatin Immunoprecipitation
- Rabbit
- Human
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Chromatin Immunoprecipitation
- 宿主：
Rabbit
- 适应物种：
Human
- 库存：
大量
- 抗原来源：
0
- 是否单克隆：
2
- 规格：
200 μg
Product Images
WB: A431 cell lysate probed with anti-NF-kappa B p65 CT (2μg/ml)
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, R, M | EMSA, IP, WB | Rabbit | Purified | Polyclonal Antibody |
Description:
Anti-NFκB p65, CT
Background Information:
The transcription factor NFκB (Nuclear Factor κB) is involved in the expression and regulation of a number of important cellular and physiological processes such as growth, development, apoptosis, immune and inflammatory response, and activation of various viral promoters including human immunodeficiency virus long terminal repeats. NFκB represents a group of structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel with five members in mammals that include Rel (cRel), RelA (p65), RelB, NFκB1 (p50 and its precursor p105), and NFκB2 (p52 and its precursor p100). NFκB/Rel proteins exist as homo- or heterodimers to form transcriptionally competent or repressive complexes. Although most NFκB dimers are activators of transcription, the p50/50 and p52/52 homodimers can repress the transcription of their target genes. The p50/p65 heterodimer of NFκB is the most abundant in cells.
Alternate Names:
- Nuclear factor NF-kappa-B p65 subunit
- v
- v-rel avian reticuloendotheliosis viral oncogene homolog A (nuclear
factor of kappa light polypeptide gene enhancer in B-cells 3 (p65)) - v-rel reticuloendotheliosis viral oncogene homolog A (avian)
- v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65
- v-rel reticuloendotheliosis viral oncogene homolog A, nuclear factor of kappa light polypeptide gene enhancer in B-cells 3, p65 (avian)
Specificity:
NFκB p65, Mr 65 kDa
Key Applications:
- Electrophoretic Mobility Shift Assay
- Immunoprecipitation
- Western Blotting
Application Notes:
Immunoprecipitation:
4 µg of a previous lot immunoprecipitated NFκB p65 from 500 μg of a human A431 carcinoma cell lysate.
4 µg of a previous lot immunoprecipitated NFκB p65 from 500 μg of a human A431 carcinoma cell lysate.
Species Reactivity:
- Human
- Rat
- Mouse
Species Reactivity Note:
Human, mouse and rat
Isotype:
IgG
Immunogen:
KLH-conjugated, synthetic peptide corresponding to the 13 C-terminal amino acids (C-DMDFSALLSQISS) of the p65 subunit of human NFκB containing an N-terminal cysteine for conjugation. The immunizing sequence is identical in mouse.
Epitope:
C-terminus
Molecular Weight:
65 kDa
Control:
TNFα treated HeLa cells, PMA, A431 cells or calcium ionophore-treated Jurkat cells
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
Presentation:
Purified rabbit polyclonal IgG in 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide and 30% glycerol. Liquid at -20°C.
Storage Conditions:
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Gene Symbol:
- RELA
- MGC131774
- NFKB3
Quality Assurance:
Routinely evaluated by Western Blot on RIPA lysates from A431 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected NFkB p65 in RIPA lysates from A431 cells.
Western Blot Analysis:
0.5-2 µg/mL of this lot detected NFkB p65 in RIPA lysates from A431 cells.
Antibody Category:
Epigenetics & Nuclear Function
Antibody Sub-Category:
Transcription Factors
Usage Statement:
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Trade Name:
Upstate (Millipore)
Concentration:
1 mg/mL
Antibody Type:
Polyclonal Antibody
Format:
Purified
Host:
Rabbit
Purification Method:
Protein A chromatography
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
peptide corresponding to the 13 C-terminal amino acids (C-DMDFSALLSQISS) of the p65 subunit of human NF κB 详细描述见链接：http://www.millipore.com/catalogue/item/06-418
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文献和实验相关实验
1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
氯化镧抑制 TNF- α诱导 NF- κ B 信号通路活化 观察氯化镧 对NF-κB信号通路中关键分子的蛋白表达和磷酸化及NF-κB／p65亚基与靶基因结合能力的影响，探索氯化镧抑制NFκB活化的可能作用位点。以1×106 个／ml接种细胞于含10％胎牛血清的RMPll640液体培养基中，于5％C02 、37℃,恒温培养，随机分组如下：①TNFα组：以含TNFα20ng／ml的无血清培养基培养Hela细胞。②氯化镧+TNF-α组：分别以含氯化镧5，25，50和100
Dual- and Triple-Color Fluorospot
of 35% ethanol for 1 min at room temperature and wash the plate five times with 200 μl of sterile water per well. b. Mix coating mAbs for IFN-γ and IL-2 in the same tube in sterile PBS, pH 7.4, at a final concentration of 15 μg/ml each. Add
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Anti-NFκB p65, CT; 200 μg
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