- 询价
- 06-314
- 2025年07月16日
- Immunoprecipitation;Western Blotting
- Rabbit
- Mouse;Monkey;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Immunoprecipitation;Western Blotting
- 宿主：
Rabbit
- 适应物种：
Mouse;Monkey;Rat
- 抗原来源：
0
- 库存：
大量
- 是否单克隆：
2
- 规格：
200 μg
23 residue, synthetic peptide (K-QYHPTDITGPLNLSDPSVSTVV) corresponding to the C-terminus of rat NR1 splice variant isoform D, with a lysine added at the N-terminus of the peptide 详细描述见链接：http://www.millipore.com/catalogue/item/06-314
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文献和实验containing chloramphenicol (Cm, 20 μg/ml)/streptomycin (Stp, 75 μg/ml) and purified using the Large construct kit (Qiagen, Valencia, CA, USA). PCR PCR reactions using oligonucleotides carrying HAs were performed using
1. Treat cells. For PC12 or NIH 3T3 cells a moderately confluent 60 mm plate is used. 2. Aspirate media. Rinse cells with PBS, aspirate.3. Lyse cells in 200 uL of SDS sample buffer (100 ℃). Immediately scrape the cells off the plate with a rubber
Dual- and Triple-Color Fluorospot
of 35% ethanol for 1 min at room temperature and wash the plate five times with 200 μl of sterile water per well. b. Mix coating mAbs for IFN-γ and IL-2 in the same tube in sterile PBS, pH 7.4, at a final concentration of 15 μg/ml each. Add
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