- 询价
- 05-613
- 2025年08月24日
- Immunoprecipitation;Western Blotting
- Mouse
- Human;Mouse;Rat
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- 详细信息
- 文献和实验
- 技术资料
- 应用范围：
Immunoprecipitation;Western Blotting
- 宿主：
Mouse
- 库存：
大量
- 适应物种：
Human;Mouse;Rat
- 抗原来源：
0
- 是否单克隆：
1
- 规格：
200 μg
Product Images
WB: A431 cell lysate was probed with anti-β-Catenin (0.5μg/ml).
| Species Reactivity | Key Applications | Host | Format | Antibody Type |
|---|---|---|---|---|
| H, M, R | IP, WB, IH | Mouse | Purified | Monoclonal Antibody |
- Immunoprecipitation
- Western Blotting
- Immunohistochemistry
- Human
- Mouse
- Rat
- CTNNB1
- CTNNB
- FLJ25606
- Beta-catenin
- PRO2286
- FLJ37923
- DKFZp686D02253
更多产品技术资讯，请访问密理博中国博客：http://blog.milliporechina.com
fusion protein corresponding to residues 1-100 of human β-Catenin 详细描述见链接：http://www.millipore.com/catalogue/item/05-613
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文献和实验7.5, 150 mM NaCl, and1% (v/v) Triton X-100. 7. Suitable SDS-PAGE and transfer setup as in Section 2.4.1 . 8. Protein Sample Loading Dye (4×) as in Section 2.4.1 . 9. Nitrocellulose or PVDF membrane. 10. Mouse anti-β-catenin antibody (available
v468. chapter 4 WNT 细胞通路 检测细胞GSK3活性与表达水平
Assays (See Note 9) 1. Pre-incubate anti-GSK3β (5 μg) monoclonal antibody withprotein G-Sepharose (400 μL of a 50% slurry) in kinasebuffer at 4°C for 1 h to conjugate the antibody to the proteinG-Sepharose beads. 2. Centrifuge the beads at 6,000× g
Expression and Preparation of Fusion Proteins from Recombinant gt1.1 Phages
-level expression, the relative stability of β-galactosidase fusion proteins, and simple approaches to purify the fusion proteins. After the desired clone is detected and purified, it is often necessary to obtain preparative amounts of recombinant protein
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