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- 详细信息
- 文献和实验
- 技术资料
- 应用范围:
Protein Purification
- 宿主:
0
- 抗原来源:
0
- 库存:
大量
- 是否单克隆:
0
- 规格:
1 x 10 L
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Product Family Information
ProSep®-vA Chromatography MediaProSep-vA chromatography media are designed to facilitate highly efficient, cost effective purification of monoclonal, polyclonal and engineered antibodies. ProSep-vA Ultra and ProSep-vA High Capacity media are manufactured using no mammalian derived products. |
更多产品技术资讯,请访问密理博中国博客:http://blog.milliporechina.com
详细描述见链接:http://www.millipore.com/catalogue/item/115115835
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文献和实验Clonality - X Chromosome Inactivation Assay
microdissected cells in 20 µl proteinase K buffer. Incubate overnight at 37°C. B: DNA Purification Lyse the cells overnight at 42°C, in 50 µl phenol:chloroform:isoamyl alcohol. Boil 10 min at 95°C. Vortex 1 min. Centrifuge 20 min at 14,000 rpm at RT
An Ultra-fast method of DNA extraction from Neurospora
) plus any other required supplements. We use a loopful of conidia from a 5-10 day old tube. Incubate at 33°C. Handle the plates carefully to prevent spillage. 2. Harvest the mycelia by filtration after 36-48 h, rinse with distilled water and remove excess water
Transformation Ultra-Competent E. coli
1. Inoculate from an overnight grown in LB. 2. Grow in 250 ml " SOB " at 18C until OD600 = 0.6. 2. On ice for 10 minutes. 3. Spin at 2500 x g (5000 rpm in a Sorvall GSA or 3000 rpm in a Beckman J-6B centrifuge) for 10 min. at 4C.
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