SILAC蛋白质组学服务

SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture) Proteomics Service

Assay Principle
Stable isotope labeling by amino acids in cell culture (SILAC) is a quantitative proteomic approach. By incorporation of arginine or lysine containing 13C or 15N into the live cell cultures, two groups of cell cultures are distinct. One group of cells grows with "light medium" essentially comprising natural isotope containing amino acids, while another grows with "heavy medium" comprising stable isotope labelled amino acids.

Diagram


Services Included
Cell Culture and Sample Preparation
Labeling incorporation control analysis
SDS-PAGE and Gel fractionation
In-gel digestion and LC-MS/MS analysis
Bioinformatic data analysis
Final report

Sample
Cell: ≥5*10^6 cell
Protein: ≥200ug (≥1ug/ul)

Applications
Study on pathogenesis
Drug target research
Protein modification research
Protein and molecular interaction
Special functional protein screening