SILAC (Stable Isotope Labeling with Amino Acids in Cell Culture) Proteomics Service
Assay Principle Stable isotope labeling by amino acids in cell culture (SILAC) is a quantitative proteomic approach. By incorporation of arginine or lysine containing 13C or 15N into the live cell cultures, two groups of cell cultures are distinct. One group of cells grows with "light medium" essentially comprising natural isotope containing amino acids, while another grows with "heavy medium" comprising stable isotope labelled amino acids.
Diagram
Services Included Cell Culture and Sample Preparation Labeling incorporation control analysis SDS-PAGE and Gel fractionation In-gel digestion and LC-MS/MS analysis Bioinformatic data analysis Final report
Applications Study on pathogenesis Drug target research Protein modification research Protein and molecular interaction Special functional protein screening