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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
-20℃,避光
- 英文名:
Dihydroethidium (Hydroethidine)
- 库存:
999
- 供应商:
Biorigin
- 规格:
5 mg
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文献和实验Fluorescence-activated flow cytometry analyses were developed for determination of reactive oxygen species (ROS) formation and membrane lipid peroxidation in live spermatozoa loaded with, respectively, hydroethidine (HE) or the lipophilic
analysis of the expression of membrane molecules on resting and Con A-activated lymphocytes using hydroethidine (a vital dye that is converted to ethidium in living cells) and MAb specific for CD3 or an activation molecule. The HE-loaded Con A blasts
Externalization(磷脂酰丝氨酸外翻):Annexin V-FITC(绿色)染细胞膜,(3)检测线粒体产生的ROS:无荧光的HE(hydroethidine,氢化乙啶)可被ROS氧化为EthBr(ethidium bromide,溴乙啡啶),发红色荧光。NAO(nonyl acridine orange,烷化吖啶橙,可发荧光)能与非氧化的cardiolipin(心磷脂,可被ROS氧化)反应而失去荧光。 (4)线粒体IMS蛋白的释放:AIF,细胞色素c,分别用荧光二抗染色。 (5)Capase








