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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃.
- 保质期:
Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.
- 英文名:
Recombinant Human Neurotensin/neuromedin N (NTS), partial
- 库存:
200
- 供应商:
武汉华美生物工程有限公司
- 规格:
20μg
英文名称:
Recombinant Human Neurotensin/neuromedin N (NTS), partial品名简称:
Recombinant Human NTS protein, partial货号:
CSB-EP016136HU规格:
20μg价格:
1344LC-MS/Western Blot:
/SDS-PAGE:
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) with 5% enrichment gel and 15% separation gel.纯度:
Greater than 90% as determined by SDS-PAGE.内毒素:
Not test生物活性:
/基因名:
NTSUniprot No.:
P30990别名:
NmN-125Neuromedin N ;NN ;NmNNeurotensin ;NTTail peptide种属:
Homo sapiens (Human)蛋白长度:
Partial来源:
E.coli分子量:
29.7 kDa表达区域:
24-143aa氨基酸序列:
SDSEEEMKALEADFLTNMHTSKISKAHVPSWKMTLLNVCSLVNNLNSPAEETGEVHEEELVARRKLPTALDGFSLEAMLTIYQLHKICHSRAFQHWELIQEDILDTGNDKNGKEEVIKRK蛋白标签:
N-terminal 6xHis-SUMO-tagged产品提供形式:
Liquid or Lyophilized powder缓冲液:
If the delivery form is liquid, the default storage buffer is Tris/PBS-based buffer, 5%-50% glycerol. If the delivery form is lyophilized powder, the buffer before lyophilization is Tris/PBS-based buffer, 6% Trehalose, pH 8.0.储存条件:
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.保质期:
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20℃/-80℃. The shelf life of lyophilized form is 12 months at -20℃/-80℃.货期:
3-7 business days注意事项:
Repeated freezing and thawing is not recommended. Store working aliquots at 4℃ for up to one week.功能1:
Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle.功能2:
Neurotensin may play an endocrine or paracrine role in the regulation of fat metabolism. It causes contraction of smooth muscle.文献:
Intermolecular interactions between the neurotensin and the third Extracellular domain loop of human neurotensin 1 receptor.Da Costa G., Bondon A., Coutant J., Curmi P., Monti J.P.J. Biomol. Struct. Dyn. 31:1381-1392(2013)研究领域:
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文献和实验Characterization of Neurotensin Receptors
48692 Binding to Cloned NTS1 Receptors in Membranes Alternate Protocol 2: Measurement of 125I‐Neurotensin Binding to Homogenates from Newborn Mouse Brain Alternate Protocol 3: Measurement of 125I‐Neurotensin
分析.4、几个概念:(1)扩增曲线 :(2) 荧光阈值:(3)Ct值:CT值的重现性:5、定量原理:理想的PCR反应: X=X0*2n非理想的PCR反应: X=X0 (1+Ex)nn:扩增反应的循环次数X:第n次循环后的产物量X0:初始模板量Ex:扩增效率5、标准曲线6、绝对定量1)确定未知样品的 C(t)值2)通过标准曲线由未知样品的C(t)值推算出其初始量二、实时荧光定量PCR的几种方法介绍 方法一:SYBR Green法 (一)工作原理 1、SYBR Green 能结合到双链DNA的小沟
GST fusion protein purification GST融合蛋白纯化
antipain " "check pH!GUSHISTOCHEMICAL STAINING1)determine number of slides needed,multiply by 0.75ml2)make up required vol of stain:for 10ml4℃ 5mg X-Gus50μl nn dimethyl formamide,dissolve10ml 50mM NaPO4 pH 73)sections best cut with vibrating knifefor
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