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- 保存条件:
所有缓冲液应密封保存,并在室温(15-25°C)下保存长达1年。具体保存情况收到产品后请按说明书存放。
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- 英文名:
Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) (50)
- 库存:
血浆/血清循环和核外RNA纯化小提试剂盒(纯化)
- 供应商:
艾美捷
- 规格:
50次
产品名称:血浆/血清循环和核外RNA纯化小提试剂盒(纯化)-Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) (50)
产品货号:51000
产品规格:50次
背景资料:血浆/血清循环和核外RNA纯化小提试剂盒(纯化)
保存建议:血浆/血清循环和核外RNA纯化小提试剂盒(纯化)-Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) (50)所有缓冲液应密封保存,并在室温(15-25°C)下保存长达1年。具体保存情况收到产品后请按说明存放。
产品描述:Norgen's Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) provides a fast, reliable and simple procedure for isolating circulating and exosomal RNA from plasma and serum samples ranging from 0.25 mL to 2 mL. Norgen's Plasma/Serum Ciruclating and Exosomal RNA Purification 96-Well Kit (Slurry Format) provides a high throughput method for isolating ciculating and exosomal RNA from plasma and serum samples using either a vacuum manifold or centrifugation. These kits are able to isolate all sizes of circulating RNA and exosomal RNA, including microRNA, without the use of phenol or chloroform. The slurry format provides an advantage over other available kits in that it does not require extension tubes for the purification of free-circulating and exosomal RNA from large sample volumes. RNA can be isolated from either fresh or frozen samples using these kits. These kits are suitable for the isolation of RNA from serum or plasma prepared from blood collected only on either EDTA or citrate. Plasma samples prepared from blood collected on heparin should not be used as heparin can significantly interfere with many downstream applications including RT-PCR.The purified plasma/serum free-circulating and exosomal RNA is eluted in an elution solution that is compatible with PCR, qPCR, methylation-sensitive reverse transcription qPCR, reverse transcription PCR, Northern blotting, RNase protection and primer extension, expression array assays, and NGS.Free-circulating and exosmal plasma and serum RNA can serve as both tumor- and fetal-specific markers for cancer detection and prenatal diagnosis. As well, free-circulating and exosomal RNAs have the potential to provide biomarkers for other disease states. Free-circulating and exosomal RNA in plasma or serum are usually present as short fragments of less than 1000nt, and free-circulating miRNA (21nt) can also be found in plasma and serum.
点击:血浆/血清循环和核外RNA纯化小提试剂盒(纯化)-Plasma/Serum Circulating and Exosomal RNA Purification Mini Kit (Slurry Format) (50)查看更详细产品说明,更多应用、储存、价格、货期等信息请致电垂询Norgen Biotek中国区域独家代理艾美捷科技有限公司。更多特色试剂盒、特色抗体以及特色试剂等产品评论,请点击查看每日生物评论网站。
Norgen Biotek成立于1998年,公司总部位于加拿大,是一家著名全球化生物技术公司,基于专利的碳化硅膜纯化技术和无苯酚专利配方,生产高品质的核酸和蛋白质纯化及富集产品用于研究和诊断。Norgen Biotek 在2003年获得加拿大国家研究委员会颁发的科技创新奖,是一家致力于样品制备并获得ISO 9001(产品质量)、ISO 13485(产品可用于商业化的医疗设备,包括用于体外诊断领域)和ISO 15189(可用于临床检测机分子诊断领域的研发能力)认证的生物科技公司。目前该公司的产品多达700余种,提供一流的样品制备试剂盒,应用于基因组和蛋白组等不同的领域。其产品主要覆盖包括各种标本类型的DNA,RNA和蛋白质的纯化富集,从活检样本(包括尿液,血浆,血清和血液)中纯化RNA和DNA,全系列的PCR试剂、缓冲液、分子量ladder和塑料过滤装置。
艾美捷科技有限公司,通过提供完整的产品和服务体系、便利的客户关系和供应链管理,为生命科学研究工作者提供整体打包方案。我们的企业的核心价值观:“激情、敬业、学习、创新、合作、卓越”。激发我们内在的创造力,提供有竞争力的生物医学产品和服务,持续为客户创造最大价值是我们的企业使命。我们的企业愿景是成为受人尊重、世界一流的生物医学产品及服务供应商。艾美捷科技与国内外优秀的生物试剂供应商保持着密切的合作关系,目前已成为众多国际知名品牌的中国总代理或一级代理,主要包括:AmyJet、AAT Bioquest、Abnova、Agrisera、Biovision、Biosensis、Cayman Chemical、Caisson Labs、Cytoskeleton、CytoNiChe、Epigentek、Equitech-Bio、Hycult Biotech、Jackson、LifeSensors、ProSpec、Norgen Biotek、Mabtech、Matreya、iRegene、StressMarq、ImmunoReagents、SouthernBiotech、Origene、US Biological、Solis BioDyne等,可以在第一时间为用户提供最前沿的专业资讯、完备的产品及物流服务。艾美捷服务升级,全方位实验服务项目上线啦~
关键词:细胞分析试剂盒,血浆/血清循环和核外RNA纯化小提试剂盒(纯化),血浆/血清循环和核外RNA纯化小提试剂盒(纯化)
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文献和实验Isolation of Cell-Free RNA from Maternal Plasma
of cell-free RNA from plasma samples, and we have found the protocol using TRIzol LS reagent (Invitrogen) as lysis buffer combined with RNeasy Mini kit (QIAGEN) to be the optimal method for extracting high-quality cell-free RNA in the highest quantities
containing100µl (6-7×108 beads/ml) of Dynabeads™ M-280 Streptaviden (Figure 1b).Pulldown (3), RNA-biotin specific Incubate the elute/Dynabead™ slurry at 4°C for 15 minutes on an orbital shaker followed by capture with a magnetic bead separator. The captured
and process following the protocol described in the PureLink™ Micro-to-Midi™ RNA Purification System manual available there . Determine the quality and quantity of the purified RNA using UV absorbance at 260 nm or Quant-iT™ RNA Assay Kit (cat
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